share
Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (13): 1559-1564. Previous Articles Next Articles
Full Papers
周华伟1, 曹洪玉1, 唐乾1, 安良梅1, 郑学仿*,1,2
投稿日期:
修回日期:
发布日期:
通讯作者:
基金资助:
国家自然基金;辽宁省高校创新团队项目;大连市科技计划项目
Zhou Huawei1; Cao Hongyu1; Tang Qian1; An Liangmei1; Zheng Xuefang*,1,2
Received:
Revised:
Published:
Contact:
Share
The photoreduction of metmyoglobin (metMb) and its effects on the changes of protein structure were investigated by UV-visible absorbance, fluorescence and circular dichroism (CD) spectra. MetMb in Na2HPO4-NaH2PO4 buffer were irradiated with xenon lamp at 430 nm and ultraviolet lamp. The results indicated that the absorbance intensity at Q band (545 nm and 580 nm) increased evidently after illumination. Through experimental verification, metMb had been reduced after the illumination. The verifying experiment included: adding sodium dithionite to metMb|adding potassium ferricyanide to the sample after the illumination|irradiating meanwhile adding CO to the sample. Through further discussion, it can be seen that the photoreduction mechanism of metMb with xenon lamp at 430 nm was photoinduced intramolecular electron transfer. In addition, the results of synchronous fluorescence and CD spectra of the sample after irradiation suggested that the polarity of microenvironment of tryptophan residues increased and the secondary structure of protein (CD data) varied with α-helices reduction from 63% to 57%, and β-sheet increase by 7%.
Key words: metmyoglobin, photoreduction, excited state, structure changes
ZHOU Hua-Wei, CAO Hong-Yu, TANG Qian, AN Liang-Mei, ZHENG Xue-Fang. Spectral Study on the Photoreduction of Metmyoglobin[J]. Acta Chimica Sinica, 2011, 69(13): 1559-1564.
Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks