Researcher cultured cells in vitro, when the concentration of cells is 10⁹/mL, gained the total proteins within cells, separated the total-proteins by using two-dimensions electrophorese SDS-PAGE (SDS polyacrylamide gel electrophoresis), and analyzed the electrophoretogram with PDQuest 7.1.1 software, then detcted the mass by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF-MS) after in-gel digestion protocol, finally, identified the expressing proteins betweem ovarian cancer parent cell COC1 and subline treated by cisplatin, to search for the molecular makers of ovarian cancer cell in the phases of cytogenesis, growth and apoptosis. Resultly, 11 proteins had matches returned from the database search leading to the identification of these proteins, only about 7 proteins [all for the ovarian cancer cell COC1 treated by DDP (cis-diamminedichloroplatinum)] have a significant difference among these proteins. Meanwhile, 4 proteins are different expression on level, 5 proteins are molecular chaperones, 4 proteins are relative to energy metabolism. Function analysis demonstrate the expression diffence, these different proteins profit to study the bio-marker of ovairian cancer.

Key words: ovarian cancer, DDP, two-dimensions electrophoresis, mass spectrometry analysis, biomarker