Thermodynamic Study on the Monolayer Mixed with Different Chol-content and PE/PC and the Morphology Observation of the Bilayers

doi:10.6023/A1012284

Acta Chimica Sinica ›› 2012, Vol. 0 ›› Issue (05): 599-605 .DOI: 10.6023/A1012284 Previous Articles Next Articles

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胆固醇与磷脂酰乙醇胺/磷脂酰胆碱混合膜的热力学特性及其单层膜形态的AFM 观察

王娟, 常怡光, 孙润广

陕西师范大学物理学与信息技术学院生物物理与生物医学工程实验室西安 710062

投稿日期:2010-12-28 修回日期:2011-10-10 发布日期:2012-03-17
通讯作者: 孙润广 E-mail:sunrunguang@snnu.edu.cn
基金资助:
国家自然科学基金项目(No. 20772077)和教育部高等学校博士学科点专项科研基金(No. 200807180001)资助项目.

Thermodynamic Study on the Monolayer Mixed with Different Chol-content and PE/PC and the Morphology Observation of the Bilayers

Wang Juan, Chang Yiguang, Sun Runguang

Laboratory of Biophysics and Biomedicine, College of Physics and Information Technology, Shaanxi Normal University, Xi'an, 710062

Received:2010-12-28 Revised:2011-10-10 Published:2012-03-17
Supported by:
Project supported by the National Natural Science Foundation of China (No. 20772077), and Research Fund for the Doctoral Program of Higher Education of China (No. 200807180001).

The effect of different cholesterol (Chol)-content on the mixed monolayer of the phosphatidylethanolamine (PE)/phosphatidylcholine (PC) (1∶1 molar ratio) has been investigated by analyzing the surface pressure-area isotherms. And the thermodynamic behavior of mixed monolayer of Chol and PE/PC (1∶1 molar ratio) has been investigated by calculating and analyzing the excess molecular area and excess Gibbs energy (ΔA_ex and ΔG_ex). The results show that Chol accelerated the phase transition and promoted the aggregation of monolayer. When X_Chol＝0.2, 0.6, 0.8, ΔA_ex and ΔG_ex displayed negative values, suggesting attractive interaction between molecules. While X_Chol＝0.4, ΔA_ex and ΔG_ex displayed positive values at the surface pressure of 15, 20, 25, 30 mN/m, indicating repelling interaction between molecules, promoting the increasing of the entropy. In addition, the deposited Langmuir-Blodgett (LB) monolayers of different ratios were investigated with atomic force microscopy (AFM).

Key words: biomembrane, the LB film technique, excess Gibbs energy, isotherm, atomic force microscopy

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Wang Juan, Chang Yiguang, Sun Runguang. Thermodynamic Study on the Monolayer Mixed with Different Chol-content and PE/PC and the Morphology Observation of the Bilayers[J]. Acta Chimica Sinica, 2012, 0(05): 599-605 .

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1 Liu, Y.-H.; Hu, L.; Xu, F.; Liu, S.; Wang, W.-B. Chin. J. Med. Phys. 2008, 25, 916 (in Chinese). (刘艳辉, 胡林, 许锋, 刘杉, 王文波, 中国医学物理学杂志, 2008, 25, 916.)

2 Hao, C.-C. M.S. Thesis, Shaanxi Normal University, Xi'an, 2007 (in Chinese). (郝长春, 硕士论文, 陕西师范大学, 西安, 2007.)

3 Longo, M. L.; Blanchette, C. D. Biochim. Biophys. Acta-Biomembanes 2010, 1798, 1357.

4 Mercado, F. V.; Maggio, B.; Wilke, N. Chem. Phys. Lipids2011, 164, 386.

5 Marques, J. T.; Viana, A. S.; De Almeida, R. F. M. Biochim. Biophys. Acta-Biomembanes 2011, 1808, 405.

6 Sui, S.-F. Molecular Membrane Biology, Higher Education Press, Beijing, 2003, pp. 7～428 (in Chinese). (隋森芳, 膜分子生物学, 高等教育出版社, 北京, 2003, pp. 7～428.)

7 Yang, F.-Y. Biomembrane, Science Press, Beijing, 2005, pp. 382～387 (in Chinese). (杨福愉, 生物膜, 科学出版社, 北京, 2005, pp. 382～387.)

8 Kirat, K. E.；Morandat, S.; Dufrene, Y. F. Biochim. Biophys. Acta 2010, 1798, 750.

9 Sun, R.-G.; Zhang, J. Atom. Mol. Phys. 2003, 20, 85 (in Chinese). (孙润广, 张静, 原子与分子物理学报, 2003, 20, 85.)

10 Sun, R.-G.; Zhang, J.; Wang, Y.-C. Prog. Biochem. Biophys. 2000, 27, 296 (in Chinese). (孙润广, 张静, 王永昌, 生物化学与生物物理进展,2000, 27, 296.)

11 Chen, Y. Y.; Sun, R. G.; Wang, B. J. Colloid Interface Sci.2011, 353, 294.

12 Garcia-Manyes, S.; Domenech, O.; Sanz, F.; Montero, M. F.; Hemandez-Borrell, J. Biochim. Biophys. Acta 2007,1768, 1191.

13 Deleu, M.; Nott, K.; Brasseur, R.; Jacques, P.; Thonart, P.; Dufrene, Y. F. Biochim. Biophys. Acta 2001, 1513, 55.

14 He, P.-S. Polymerization in Two-dimensional State Polymerization in Monolayer and Langmuir-Blodgett Film, Press of University of Science and Technology of China, Hefei,2008, pp. 6～53 (in Chinese). ( 何平笙, 二维状态下的聚合- 单分子膜和 Langmuir-Blodgett 膜的聚合, 中国科学技术大学出版社, 合肥, 2008, pp. 6～53.)

15 Yang, X.-L.; Sun, R.-G.; Zhang, J. Chin. J. Liquid Crystals Displays 2006, 21, 349 (in Chinese). (杨小乐, 孙润广, 张静, 液晶与显示, 2006, 21, 349.)

16 Hao, C.-C; Sun, R.-G. Atom. Mol. Phys. 2007, 24, 932 (in Chinese). (郝长春, 孙润广, 原子与分子物理学报, 2007, 24, 932.)

17 Serfis, A. B.; Brancato, S.; Fiesler, S. J. Biochim. Biophys. Acta 2001, 1511, 342.

18 Nichols-Smith, S.;The, S. Y.; Kuhl, T. L. Biochim. Biophys. Acta 2004, 1663, 82.

19 Domenech, O.; Sanz, F.; Montero, M. F.; Hemandez- Borrell, J. Biochim. Biophys. Acta 2006, 1758, 213.

20 Sun, R.-G.; Hao, C.-C.; Chang, Y.-G.; Zhang, J.; Niu, C.-L. Acta Chim. Sinica 2009, 67, 1808 (in Chinese). (孙润广, 郝长春, 常怡光, 张静, 牛春玲, 化学学报,2009, 67, 1808.)

21 Chang, Y.-G.; Sun, R.-G.; Hao, C.-C. Chem. J. Chin. Univ.2010, 31, 559 (in Chinese). (常怡光, 孙润广, 郝长春, 高等学校化学学报, 2010, 31,559.)

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胆固醇与磷脂酰乙醇胺/磷脂酰胆碱混合膜的热力学特性及其单层膜形态的AFM 观察

Thermodynamic Study on the Monolayer Mixed with Different Chol-content and PE/PC and the Morphology Observation of the Bilayers

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