Study of the Aggregates in Lysozyme Solution before Crystal Growth

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (8): 757-761. Previous Articles Next Articles

溶菌酶晶体生长前期溶液中聚集体研究

戴国亮, 于泳, 康琦, 胡文瑞

中国科学院力学研究所, 国家微重力实验室, 北京, 100080

投稿日期:2003-09-04 修回日期:2003-12-26 发布日期:2014-02-18
通讯作者: 戴国亮,E-mail:dspr@imech.ac.cn;Tel:010-62543864;Fax:010-62615524. E-mail:dspr@imech.ac.cn
基金资助:
国家自然科学基金(No.30270302)和中国科学院知识创新工程重要方向(Nos.KSCX2-SW-322,KSCX2-SW-L05)资助项目.

Study of the Aggregates in Lysozyme Solution before Crystal Growth

DAI Guo-Liang, YU Yong, KANG Qi, HU Wen-Rui

National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080

Received:2003-09-04 Revised:2003-12-26 Published:2014-02-18

Abstract

The aggregates in lysozyme solution with different NaCl concentrations in which nuclei have not appeared were studied by dynamic light scattering (DLS) method. In addition, the aggregates absorbed on the silicon wafer surface were observed by atomic force microscope (AFM). The results showed that when the NaCl concentration was lower than 0.4 mol·L^-1, the higher the NaCl concentration was, the smaller the dimension of the aggregates was. The big aggregates disappeared and only aggregates with the dimension of 3～4 nm in lysozyme solution remained when NaCl concentration was 0.4 or 0.5 mol·L^-1. The result of Zeta potential showed the change of the interaction between NaCl and lysozyme molecules. From the experimental results, we conclude that lysozyme crystal could not grow in solution in the presence of amorphous aggregates bigger than 100 nm in dimension. Additionally, the difference between results of DLS and AFM was analyzed.

Key words: dynamic light scattering, atomic force microscope, crystal growth, lysozyme, aggregate

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DAI Guo-Liang, YU Yong, KANG Qi, HU Wen-Rui. Study of the Aggregates in Lysozyme Solution before Crystal Growth[J]. Acta Chimica Sinica, 2004, 62(8): 757-761.

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溶菌酶晶体生长前期溶液中聚集体研究

Study of the Aggregates in Lysozyme Solution before Crystal Growth

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