Effect of Extraneous Lanthanum(Ⅲ) on the Structure of Horseradish Peroxidase

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (14): 1318-1322. Previous Articles Next Articles

外源镧(Ⅲ)对辣根过氧化物酶的结构的影响

夏炳乐, 彭敦耕, 郑晓云, 肖厚荣, 盛良全, 刘清亮

中国科学技术大学化学系, 合肥, 230026

投稿日期:2003-05-07 修回日期:2004-03-17 发布日期:2014-02-17
通讯作者: 夏炳乐,E-mail:xiabl@ustc.edu.cn;彭敦耕,E-mail:dgpeng@ustc.edu E-mail:xiabl@ustc.edu.cn;dgpeng@ustc.edu
基金资助:
国家烟草专卖局科研基金(No.1002001041)资助项目.

Effect of Extraneous Lanthanum(Ⅲ) on the Structure of Horseradish Peroxidase

XIA Bing-Le, PENG Dun-Geng, ZHENG Xiao-Yun, XIAO Hou-Rong, SHENG Liang-Quan, LIU Qing-Liang

Department of Chemistry, University of Science and Technology of China, Hefei 230026

Received:2003-05-07 Revised:2004-03-17 Published:2014-02-17

Abstract

Through the fluorescence spectra, FT-IR spectra and curve-fit, the effect of lanthanum(Ⅲ) on horseradish peroxidase (HRP) was studied.It is concluded that the reason of the stimulation is due to the binding of La(Ⅲ) to HRP, which induced the changes of the enzyme's secondary structure and the microenvironment of the fluorophore, while adding La(Ⅲ) into the HRP.The activation mechanism of La(Ⅲ) to the HRP was also discussed.

Key words: horseradish peroxidase, lanthanum(Ⅲ), binding, microenvironment, secondary structure

Cite this article

XIA Bing-Le, PENG Dun-Geng, ZHENG Xiao-Yun, XIAO Hou-Rong, SHENG Liang-Quan, LIU Qing-Liang. Effect of Extraneous Lanthanum(Ⅲ) on the Structure of Horseradish Peroxidase[J]. Acta Chimica Sinica, 2004, 62(14): 1318-1322.

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外源镧(Ⅲ)对辣根过氧化物酶的结构的影响

Effect of Extraneous Lanthanum(Ⅲ) on the Structure of Horseradish Peroxidase

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