Dual-Mode Sensing of Biomarkers by Mimic Enzyme-Natural Enzyme Cascade Signal Amplification

doi:10.6023/A20030079

Acta Chimica Sinica ›› 2020, Vol. 78 ›› Issue (5): 419-426.DOI: 10.6023/A20030079 Previous Articles Next Articles

Special Issue: 分子探针、纳米生物学与生命分析化学

Article

基于模拟酶-天然酶级联反应的双模式传感平台用于生物标志物的超灵敏检测

樊蕾, 江群英, 潘敏, 王文晓, 张丽, 刘晓庆

武汉大学化学与分子科学学院武汉 430072

投稿日期:2020-03-19 发布日期:2020-05-06
通讯作者: 刘晓庆 E-mail:xiaoqingliu@whu.edu.cn
基金资助:
项目受国家自然科学基金（No.81602610）和中央高校基本科研基金（No.2042018kf1006）资助.

Dual-Mode Sensing of Biomarkers by Mimic Enzyme-Natural Enzyme Cascade Signal Amplification

Fan Lei, Jiang Qunying, Pan Min, Wang Wenxiao, Zhang Li, Liu Xiaoqing

College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072

Received:2020-03-19 Published:2020-05-06
Supported by:
Project supported by the National Natural Science Foundation of China (No. 81602610) and the Fundamental Research Funds for the Central Universities (No. 2042018kf1006).

Highly sensitive and accurate analysis of significant biomarkers such as alkaline phosphatase (ALP) is essential for early detection and treatment of diseases. In this work, a fluorescence/UV-vis dual-mode sensing platform was constructed for amplified detection of ALP and pyrophosphate ion (PPi) based on mimic enzyme-natural enzyme cascade reactions. Cu-Based metal-organic frameworks HKUST-1 which possesses of oxidase-like activity and can effectively catalyze the oxidation of indicator o-phenylenediamine (OPD) by the surface-active sites were prepared. The oxidation products of OPD exhibit strong UV-vis absorption and fluorescent signals at 416 and 568 nm, respectively. After adding PPi, the catalytic activity of HKUST-1 was selectively inhibited due to the combination of PPi with Cu²⁺ on the surface of HKUST-1, that resulted in fluorescence and UV-vis signal reducing. Once ALP was introduced into the system, PPi can be specifically hydrolyzed into phosphate ions (Pi), and the oxidase-like activity of HKUST-1 recovered. Thus, the fluorescent and UV-vis signals were regenerated by an ALP-triggered mimic enzyme-natural enzyme cascade reaction. On account of the inhibition of oxidase-like activity of HKUST-1 by PPi and the recovery by ALP, an ultrasensitive dual-mode sensing platform of biomarkers based on mimic enzyme-natural enzyme cascade reactions has been developed. Under optimal conditions, the linear range of ALP by fluorescence/UV-vis detection is 0.02~3.5 and 0.04~3.5 nmol·L^-1, and the detection limit of fluorescence and UV-vis assay is as low as 0.0078 and 0.039 nmol·L^-1, respectively. As far as we know, it is the first time that the mimic enzyme-natural enzyme cascade reaction is applied to dual-mode bioanalysis. Due to the enzyme cascade amplification and dual-mode signal output, this developed strategy has the advantages of high sensitivity, low detection limit, high accuracy and reliability, and can realize ultrasensitive analysis of ALP in human serum samples, which shows great potential for clinical diagnosis.

Key words: biomarker detection, alkaline phosphatase, dual-mode detection, mimic enzyme, biosensing

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Fan Lei, Jiang Qunying, Pan Min, Wang Wenxiao, Zhang Li, Liu Xiaoqing. Dual-Mode Sensing of Biomarkers by Mimic Enzyme-Natural Enzyme Cascade Signal Amplification[J]. Acta Chimica Sinica, 2020, 78(5): 419-426.

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基于模拟酶-天然酶级联反应的双模式传感平台用于生物标志物的超灵敏检测

Dual-Mode Sensing of Biomarkers by Mimic Enzyme-Natural Enzyme Cascade Signal Amplification

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