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Acta Chimica Sinica >

2012 , Vol. 70 >Issue 11: 1304 - 1308

DOI: https://doi.org/10.6023/A1201033

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Detection for Polyketide Synthase Gene of Aspergillus ochraceus based on Au Nanoparticles Enhancing SPR Biosensor

  • Li Ying ,
  • Lin Zhao ,
  • Li Rongzhuo ,
  • Liu Xia
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  • College of Food Science and Technology, Hunan Agricultural University, Hunan Province Key Laboratory of Food Science and Biotechnology, Changsha 410128

Received date: 2012-01-03

  Revised date: 2012-03-06

  Online published: 2012-03-09

Supported by

Project supported by the Scientific Research Fund of Hunan Provincial Education Department (No. 10B050), Key Project of Science and Technology Plan of Changsha (No. K1005181-21) and Graduate Innovation Fund of Hunan Province (No. CX2010B281).

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Abstract

The surface plasmon resonance (SPR) method is established for indirect detection of the ochratoxin A. The 25-base oligonucleotide sequence of polyketide synthase (PKS) gene which is expressed during early stage of ochratoxin A synthesis, was detected respectively using direct assay and Au nanoparticles as sensing membrane assay based on the two channels surface plasmon resonance (SPR) biosensor. At the same time, influence of 6-mercapto-1-hexanol (MCH) blocking on SPR signal was also studied. As a result, the limit of detection (LOD) of the direct assay was 12.5 nmol/L. And the SPR signal was enhanced with 6-mercapto-1-hexanol (MCH) blocking. With Au nanoparticles as a sensing membrane, LOD was 0.25 nmol/L, sensitivity was 50 times higher than that of direct detection. SPR signal could be dramatically enhanced with Au nanoparticles as sensing membrane instead of as label, and the operation was simple.

Key words： surface plasmon resonance (SPR); Au nanoparticles; ochratoxin A; polyketide synthase (PKS)

Cite this article

Li Ying , Lin Zhao , Li Rongzhuo , Liu Xia . Detection for Polyketide Synthase Gene of Aspergillus ochraceus based on Au Nanoparticles Enhancing SPR Biosensor[J]. Acta Chimica Sinica, 2012 , 70(11) : 1304 -1308 . DOI: 10.6023/A1201033

References

1 Betina, V. Mycotoxins: Chemical, Biological and Environmental Aspects, Elsevier, Amsterdam, 1989, 438.  

2 Turner, N. W.; Subrahmanyam, S.; Piletsky, S. A. Anal. Chim. Acta 2009, 632, 168.  

3 Yu, J. C. C.; Lai, E. P. C. Synth. Met. 2004, 143, 253.  

4 Fu, X. H. Anal. Lett. 2007, 40, 2641.  

5 Yuan, J.; Deng, D.; Lauren, D. R.; Aguilar, M.; Wu, Y. Anal. Chim. Acta 2009, 656, 63.  

6 Moss, M. O. Food Addit. Contam. (Suppl.) 1996, 13, 5.  

7 Moss, M. O. Symp. Ser. Soc. Appl. Microbiol. 1998, 27,62S.

8 O’Callaghan, J.; Caddick, M. X.; Dobson, A. D. W. Microbiology2003, 149, 3485.  

9 Atoui, A.; Dao, H. P.; Mathieu, F.; Lebrihi, A. Mol. Nutr. Food Res. 2006, 50, 488.  

10 Culebras, P. V. M.; Crespo-Sempere, A.; Gil, J. V.; Ramon, D. Food Sci. Technol. Int. 2009, 15, 97.  

11 Xiao, S.-B. J. Maize Sci. 2009, 17(2), 38 (in Chinese). (肖守斌, 玉米科学, 2009, 17(2), 38.)  

12 Joung, H.-A.; Lee, N.-R.; Lee, S. K.; Ahn, J. Anal. Chim. Acta 2008, 63, 168.

13 Jordan, C. E.; Frutos, A. G.; Thiel, A. J.; Cron, R. M. Anal. Chem. 1997, 69, 4939.  

14 Taylor, A. D.; Ladd, J.; Yu, Q. M.; Chen, S. F.; Homola, J.; Jiang, S. Y. Biosens. Bioelectron. 2006, 22, 752.  

15 Golden, M. S.; Bjonnes, A. C.; Georgiadis, R. M. J. Phys. Chem. 2010, 114, 8837.

16 Hu, R.-S.; Liu, S.-T.; Zhu, T.; Liu, Z.-F.; Huang, X.-H.; Huang, H.-Z. Acta Phys.-Chim. Sin. 1999, 15(11), 961 (in Chinese). (胡瑞省, 刘善堂, 朱涛, 刘忠范, 黄小华, 黄惠忠, 物理 化学学报, 1999, 15(11), 961.)

17 Wu, J.; Liu, Y.-G.; Jia, Q.; Zhang, J.-L.; Jiang, Y. J. Southwest Univ. Nationalities (Humanities Soc. Sci.) 2005, 46(4), 46 (in Chinese). (吴皎, 刘亚刚, 贾清, 张金灵, 姜宇, 西南民族大学学 报, 2005, 46(4), 46.)  

18 Wang, R.; Tombelli, S.; Minunni, M.; Spiriti, M. M.; Mascini, M. Biosens. Bioelectron. 2004, 20, 967.  

19 Milkani, E.; Morais, S.; Lambert, C. R.; McGimpsey, W. G. Biosens. Bioelectron. 2010, 25, 1217.  

20 Levicky, R.; Herne, T. M.; Tarlov, M. J.; Satija, S. K. J. Am. Chem. Soc. 1998, 120(38), 9787.  

21 Kim, S.-H.; Lee, J.-Y.; Lee, S.-J.; Lee, H.-J. Talanta 2010,81, 1755.  

22 Wang, Q. Ph.D. Dissertation, Hunan University, Changsha,2007, pp. 62～67 (in Chinese). (王青, 博士论文, 湖南大学, 长沙, 2007, pp. 62～67.)

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