Rheological Properties, Drug Release Behavior and Cytocompatibility of Novel Hydrogels Prepared from Carboxymethyl Chitosan
Received date: 2014-10-14
Online published: 2014-12-09
Supported by
Project supported by the National Natural Science Foundation of China (Nos. 50873030, 51073041).
Hydrogels were prepared by crosslinking carboxymethyl chitosan (CMCS) using 1-ethyl-3-(3-dimethylamino- propyl)-1-carbodiimide/N-hydroxysuccinimide (EDC/NHS) as catalyst under mild conditions. The resulting hydrogels present different crosslinking density, depending on the amount of EDC/NHS. Rheological studies show that hydrogels with high crosslinking density present higher storage modulus than those with low crosslinking density. A model drug, thymopentin (TP-5) is loaded in CMCS hydrogels. Parabolic release profiles are obtained in all cases. High crosslinking density hydrogels present slower release rate as compared to low crosslinking density ones because the former exhibits more compact structure. Preliminary studies were performed to evaluate the cytotoxicity of CMCS hydrogels by using MTT assay. Both cell morphology and RGR results show that the CMCS hydrogels present very low toxicity. Therefore, CMCS hydrogels are promising for applications in the fields of tissue engineering and controlled drug delivery.
Liu Shuilian , Zhou Yang , Chen Fuhua , Zhu Shoujin , Su Feng , Li Suming . Rheological Properties, Drug Release Behavior and Cytocompatibility of Novel Hydrogels Prepared from Carboxymethyl Chitosan[J]. Acta Chimica Sinica, 2015 , 73(1) : 47 -52 . DOI: 10.6023/A14100710
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