Rheological Properties, Drug Release Behavior and Cytocompatibility of Novel Hydrogels Prepared from Carboxymethyl Chitosan

doi:10.6023/A14100710

Acta Chim. Sinica ›› 2015, Vol. 73 ›› Issue (1): 47-52.DOI: 10.6023/A14100710 Previous Articles Next Articles

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新型羧甲基壳聚糖水凝胶流变性能,药物释放及细胞相容性研究

刘水莲^a, 周洋^a, 陈福花^b, 朱寿进^a, 宿烽^a, 李速明^c

a 青岛科技大学高性能聚合物研究院青岛 266042;
b 青岛中化新材料实验室青岛 266042;
c 蒙彼利埃二大欧洲薄膜研究院法国蒙彼利埃 34095

投稿日期:2014-10-14 发布日期:2014-12-09
通讯作者: 宿烽, 李速明 E-mail:qdkdsufeng@hotmail.com;lisuming@hotmail.com
基金资助:
项目受国家自然科学基金(Nos. 50873030, 51073041)资助.

Rheological Properties, Drug Release Behavior and Cytocompatibility of Novel Hydrogels Prepared from Carboxymethyl Chitosan

Liu Shuilian^a, Zhou Yang^a, Chen Fuhua^b, Zhu Shoujin^a, Su Feng^a, Li Suming^c

a Institute of High Performance Polymer, Qingdao University of Science and Technology, Qingdao 266042;
b Qingdao Sinochem New Materials Laboratory, Qingdao 266042;
c Institut Europeen des Membranes, UMR CNRS 5635, Universite Montpellier II, Montpellier 34095, France

Received:2014-10-14 Published:2014-12-09
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 50873030, 51073041).

1. Rheological Properties, Drug Release Behavior and Cytocompatibility of Novel Hydrogels Prepared from Carboxymethyl Chitosan.PDF(158KB)

Abstract

Hydrogels were prepared by crosslinking carboxymethyl chitosan (CMCS) using 1-ethyl-3-(3-dimethylamino- propyl)-1-carbodiimide/N-hydroxysuccinimide (EDC/NHS) as catalyst under mild conditions. The resulting hydrogels present different crosslinking density, depending on the amount of EDC/NHS. Rheological studies show that hydrogels with high crosslinking density present higher storage modulus than those with low crosslinking density. A model drug, thymopentin (TP-5) is loaded in CMCS hydrogels. Parabolic release profiles are obtained in all cases. High crosslinking density hydrogels present slower release rate as compared to low crosslinking density ones because the former exhibits more compact structure. Preliminary studies were performed to evaluate the cytotoxicity of CMCS hydrogels by using MTT assay. Both cell morphology and RGR results show that the CMCS hydrogels present very low toxicity. Therefore, CMCS hydrogels are promising for applications in the fields of tissue engineering and controlled drug delivery.

Key words: carboxymethyl chitosan, hydrogel, rheological properties, drug release, cytocompatibility

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Liu Shuilian, Zhou Yang, Chen Fuhua, Zhu Shoujin, Su Feng, Li Suming. Rheological Properties, Drug Release Behavior and Cytocompatibility of Novel Hydrogels Prepared from Carboxymethyl Chitosan[J]. Acta Chim. Sinica, 2015, 73(1): 47-52.

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新型羧甲基壳聚糖水凝胶流变性能,药物释放及细胞相容性研究

Rheological Properties, Drug Release Behavior and Cytocompatibility of Novel Hydrogels Prepared from Carboxymethyl Chitosan

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