Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol
Received date: 2018-10-17
Online published: 2019-01-17
Supported by
Project supported by the Scientific Research Fund of Hunan Provincial Education Department (No. 17C0033).
A label free and rapid fluorescent method for quantitative detection of chloramphenicol (CAP) based on graphene oxide (GO) fluorescence functional G-quadruplex probe (FGP) was developed. The FGP consisted of a choramphenicol aptamer and a G-rich sequence. The aptamer was used to bind CAP and the G-quadruplex formed by G-rich sequence was employed as a signal reporter after binding to Thioflavin T (ThT). In the absence of CAP, the FGP was absorbed onto the surface of GO through π-π stacking interactions, which restrained the G-rich sequence to form a G-quadruplex structure. Thus, the fluorescent intensity of background was low. In the addition of the CAP, the aptamer part of FGP could recognize and bind CAP to form a target-FGP complex, which led to the desorption of the complex from GO. Therefore, the free G-rich sequence could form G-quadruplex structure and bind to ThT, resulting a increase in the fluorescence intensity of the solution. We observed that the fluorescence increasement of the sensing platform had a linear relationship with the concentrations of CAP in the range of 2~20 nmol/L, and the limit of detection was 1.45 nmol/L. Besides, this detection system was applied for detecting CAP in the spiked milk, the recovery rate was between 93.2%~103.3%. These results indicated that this developed method can be used to efficiently recognize CAP in real samples.
Key words： aptamer; fluorescence; chloramphenicol; G-quadruplex; graphene oxide
Lu Jinghe , Tan Shuzhen , Zhu Yuqing , Li Wei , Chen Tianxiao , Wang Yao , Liu Chen . Fluorescent Aptamer-functionalized Graphene Oxide Biosensor for Rapid Detection of Chloramphenicol[J]. Acta Chimica Sinica, 2019 , 77(3) : 253 -256 . DOI: 10.6023/A18100433
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