Determination of β-Glucosidase Activity Based on Enzyme-Triggered Click Chemistry

doi:10.6023/cjoc201803011

Chin. J. Org. Chem. ›› 2018, Vol. 38 ›› Issue (10): 2775-2779.DOI: 10.6023/cjoc201803011 Previous Articles Next Articles

Notes

基于酶触发的点击化学反应测定β-葡萄糖苷酶活性

王龙文, 马济美, 程鑫, 李子龙, 孙林皓, 曾贞, 江洪

华中农业大学理学院武汉 430070

收稿日期:2018-03-09 修回日期:2018-04-21 发布日期:2018-06-01
通讯作者: 马济美,E-mail:majimei@mail.hzau.edu.cn;江洪,E-mail:jianghong0066@126.com E-mail:majimei@mail.hzau.edu.cn;jianghong0066@126.com
基金资助:
国家自然科学基金（No.21402056）资助项目.

Determination of β-Glucosidase Activity Based on Enzyme-Triggered Click Chemistry

Wang Longwen, Ma Jimei, Cheng Xin, Li Zilong, Sun Linhao, Zeng Zhen, Jiang Hong

Department of Chemistry, College of Science, Huazhong Agricultural University, Wuhan 430070

Received:2018-03-09 Revised:2018-04-21 Published:2018-06-01
Contact: 10.6023/cjoc201803011 E-mail:majimei@mail.hzau.edu.cn;jianghong0066@126.com
Supported by:
Project supported by the National Natural Science Foundation of China (No. 21402056).

1. Determination of β-Glucosidase Activity Based on Enzyme-Triggered Click Chemistry.PDF(1059KB)

Abstract

A novel method to quantify β-glucosidase activity was developed by coupling the cleavage of 2-O-(β-glucopyra-nosyl)ascorbic acid with the reduction of Cu (Ⅱ). The in situ generated Cu (I) catalyzed the cycloaddition between weakly fluorescent coumarin and benzyl azide to yield a highly fluorescent triazole product. The fluorescence intensity was dependently enhanced on the increase of the activity of β-glucosidase and a linear relationship was found between 1～40 U/L. This cascade allows detection of β-glucosidase with a limit of detection of 0.456 U/L.

Key words: β-glucosidase activity, determination, 2-O-(β-glucopyranosyl)ascorbic acid, click chemistry

Cite this article

Wang Longwen, Ma Jimei, Cheng Xin, Li Zilong, Sun Linhao, Zeng Zhen, Jiang Hong. Determination of β-Glucosidase Activity Based on Enzyme-Triggered Click Chemistry[J]. Chin. J. Org. Chem., 2018, 38(10): 2775-2779.

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基于酶触发的点击化学反应测定β-葡萄糖苷酶活性

Determination of β-Glucosidase Activity Based on Enzyme-Triggered Click Chemistry

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