Researches on the Subergane-Type Sesquiterpenes from the Soft Coral-Derived Fungus Aspergillus sp. EGF15-0-3

doi:10.6023/cjoc201911040

Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (5): 1275-1280.DOI: 10.6023/cjoc201911040 Previous Articles Next Articles

软珊瑚共附生真菌Aspergillus sp.EGF15-0-3的柳珊瑚酸类代谢产物研究

冯婵^a, 韦霞^a, 胡金姗^a, 王思玉^a, 刘炳新^a, 谢珍玉^b, 荣莉^a, 李小翚^a, 张翠仙^a

a 广州中医药大学中药学院广州 510006;
b 海南大学药学院海口 570228

收稿日期:2019-11-30 修回日期:2020-01-13 发布日期:2020-02-15
通讯作者: 李小翚, 张翠仙 E-mail:zhangcuixian@gzucm.edu.cn;lxh@gzucm.edu.cn
基金资助:
国家自然科学基金（No.81741160）、广东省自然科学基金（No.2014A030313411）、广东省科技计划（Nos.2017A020217008，2015A020216017）、广东省自然资源厅广东省海洋经济发展海洋六大产业专项资金（No.粤自然资合[2020]039）、广东省中药药效物质基础和创新药物研究重点实验室开放课题、广州中医药大学薪火计划重点项目（No.XH20170110）和海南大学南海海洋资源利用国家重点实验室开放课题（No.2016003）资助项目.

Researches on the Subergane-Type Sesquiterpenes from the Soft Coral-Derived Fungus Aspergillus sp. EGF15-0-3

Feng Chan^a, Wei Xia^a, Hu Jinshan^a, Wang Siyu^a, Liu Bingxin^a, Xie Zhenyu^b, Rong Li^a, Li Xiaohui^a, Zhang Cuixian^a

a School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou 510006;
b College of Marine Science, Hainan University, Haikou 570228

Received:2019-11-30 Revised:2020-01-13 Published:2020-02-15
Supported by:
Project supported by the National Natural Science Foundation of China (No.81741160), the Natural Science Foundation of Guangdong Province (No. 2014A030313411), the Science and Technology Planning Project of Guangdong Province (Nos. 2017A020217008, 2015A020216017), the Special Project for Marine Economic Development (Six Major Marine Industries) of Guangdong Province of Department of Natural Resources of Guangdong Province (No. [2020]039), the Open Subject of Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, the Fire Plan Project of the Guangzhou University of Chinese Medicine (No. XH20170110), and the Open Subject of State Key Laboratory of Marine Resources Utilization in the South China Sea of Hainan University (No. 2016003).

Subergorgic acid (1), a cyclopentapentalane sesquiterpenoid and potential reversible cholinesterase inhibitor, and its analogue 2-deoxy-2β-hydroxysubergorgic acid (2) were first isolated from soft coral-associated symbiotic and epiphytic fungus Aspergillus sp. EGF15-0-3. Their structures were established by NMR and HRESIMS spectroscopic data. According to the strategy of one strain many compounds (OSMAC) and eutrophic culture, Global Natural Products Molecular Networking (GNPS) was applied to detect the presence of compounds 1 and 2 in 23 different media for optimal medium compositions. As a result, compound 1 was found in 6 different media besides potato dextrose broth (PDB) medium, the contents of 2 were different in the solid and liquid media, and the best medium for compounds 1 and 2 was GPY+CaCO₃. It was demonstrated that the soft coral-associated symbiotic and epiphytic fungus Aspergillus sp. EGF15-0-3 was the microbiological producer of compounds 1 and 2. This work provided strong evidence to support the hypothesis that novel and bioactive secondary metabolites produced by marine invertebrates often come from endogenetic, epiphytic, symbiotic or environmental microorganisms.

Key words: soft coral-associated symbiotic and epiphytic fungus, Aspergillus sp.EGF15-0-3, subergorgic acid, GNPS

Cite this article

Feng Chan, Wei Xia, Hu Jinshan, Wang Siyu, Liu Bingxin, Xie Zhenyu, Rong Li, Li Xiaohui, Zhang Cuixian. Researches on the Subergane-Type Sesquiterpenes from the Soft Coral-Derived Fungus Aspergillus sp. EGF15-0-3[J]. Chinese Journal of Organic Chemistry, 2020, 40(5): 1275-1280.

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软珊瑚共附生真菌Aspergillus sp.EGF15-0-3的柳珊瑚酸类代谢产物研究

Researches on the Subergane-Type Sesquiterpenes from the Soft Coral-Derived Fungus Aspergillus sp. EGF15-0-3

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