Application of an Intelligent Integrated Platform Based on “OFF-ON” Fluorescent Probe in Salmon Freshness Monitoring

doi:10.6023/cjoc202507024

Chinese Journal of Organic Chemistry Previous Articles Next Articles

ARTICLE

基于“OFF-ON”型荧光探针的智能一体化平台在三文鱼肉新鲜度监测中的应用

孙小飞^a,b,c, 张海媛^a, 李娟^a, 马骥^b, 王若溪^a, 钟克利^a, 李学鹏^a, 梁天宇^a, 汤立军^a,*, 励建荣^a

^a渤海大学食品科学与工程学院化学与材料工程学院海洋研究院生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁锦州 121013;
^b佛山大学广东省食品智能制造重点实验室广东佛山 528225;
^c新加坡国立大学理学院食品科学与技术系新加坡 117542

收稿日期:2025-07-19 修回日期:2025-09-07
基金资助:
国家自然科学基金青年科学基金项目（32201948）; 广东省食品智能制造重点实验室开放基金（2022B1212010015,GPKLIFM-KF-202410）; 国家留学基金管理委员会和辽宁省教育厅地方合作项目地方创新子项目（202308210400）; 广东省水产品加工与安全重点实验室开放基金（GDPKLAPPS2404）; 辽宁省教育厅项目（LJ212510167023）.

Application of an Intelligent Integrated Platform Based on “OFF-ON” Fluorescent Probe in Salmon Freshness Monitoring

Sun Xiaofei^a,b,c, Zhang Haiyuan^a, Li Juan^a, Ma Ji^b, Wang Ruoxi^a, Zhong Keli^a, Li Xuepeng^a, Liang Tianyu^a, Tang Lijun^a,*, Li Jianrong^a,*

^aBohai University, College of Food Science and Engineering, College of Chemistry and Materials Engineering, Institute of Oceanography,National and Local Joint Engineering Research Center for Storage, Processing and Safety Control Technology of Fresh Agricultural Products Liao Ning 121013;
^bFoshan University, Guangdong Provincial Key Laboratory of Food Intelligent Manufacturing,GuangDong, 528225;
^cNational University of Singapore, College of Science, Department of Food Science and Technology, Singapore, 117542

Received:2025-07-19 Revised:2025-09-07
Contact: *E-mail: ljtang@bhu.edu.cn;lijr6491@163.com
Supported by:
National Natural Science Foundation of China Youth Science Fund Project (32201948); Open Fund of Guangdong Provincial Key Laboratory of Food Intelligent Manufacturing (2022B1212010015, GPKLIFM-KF-202410); Local Innovative Sub-project of the Local Cooperation Project of the National Scholarship Council of China and Liaoning Provincial Department of Education (202308210400); Guangdong Province Open Fund for Key Laboratory of Aquatic Product Processing and Safety (GDPKLAPPS2404); the Scientific Research Fund of Liaoning Provincial Education Department (No. LJ212510167023).

1. .pdf(664KB)

Abstract

Fish freshness is closely related to food safety and human health. Therefore, it is of great significance to develop a rapid, simple, non-destructive and intuitive fish freshness detection method. Herein, an “OFF-ON” fluorescent probe PTD was successfully synthesized by using thiophenealdehyde and 2,4-thiazolidinedione through Knoevenagel condensation. The probe had dual recognition capability of colorimetric and fluorescence for amines in solution, with high sensitivity (1.20 μM) and fast response speed (3 s). The prepared PTD-loaded indicator label exhibited dual-channel response to the salmon freshness, with the daylight color changing from orange to earth-yellow and the fluorescence shifting from bright orange to flesh pink. By utilizing smartphone software and the indicator label, an intelligent integrated detection platform was constructed, which could automatically output the total volatile basic nitrogen (TVB-N) value of salmon. Under room temperature condition, its accuracy and reliability were confirmed by the Chinese national standard method. This study is expected to provide new ideas for portable intelligent detection fields and offer a smart reading tool for assessing fish freshness to merchants, consume

Key words: Fluorescent probe, indicator label, intelligent integrated platform, freshness

Cite this article

Sun Xiaofei, Zhang Haiyuan, Li Juan, Ma Ji, Wang Ruoxi, Zhong Keli, Li Xuepeng, Liang Tianyu, Tang Lijun, Li Jianrong. Application of an Intelligent Integrated Platform Based on “OFF-ON” Fluorescent Probe in Salmon Freshness Monitoring[J]. Chinese Journal of Organic Chemistry, doi: 10.6023/cjoc202507024.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

share this article

References

[1] Gu M. H.; Zheng X. C.; Li C.; Zheng Z.; Song G.; Xu L.; Chen L.; Zhang D.Nano Today 2025, 62, 102675.
[2] Calabretta M. M.; Gregucci D.; Desiderio R.; Michelini E.Biosensors 2023, 13, 126.
[3] Xu F. Z.; Wang C. Y.; Wang Q.; Zou J.; Qiao Y.; Guo Z.; Zhao W.; Zhu W. Chem. Commun2022, 58, 6393-6396.
[4] Bu L. L.; Liu D. F.; Wang L. D.; Sun M.; Gao C.; Xie Y. CCL2024, 35, 109290.
[5] Jiang L. R.; Ye H.; Ma D.; Rodrigues J.; Sheng R.; Min D. Analyst2022, 147, 923-931.
[6] Sun X. F.; Jiang X.; Zhao X. Y.; Wang Z.; Yuan J.; Li Y.; Li X.; Li J.; Tang L.; Zhong K. Food Chem2025, 473, 143013.
[7] Zhang J.; Wang N.; Ji X.; Tao Y.; Wang J.; Zhao W.Chem 2020, 26, 4172.
[8] Liu Y.; Luo L.; Huang G.; Zhou K.; Xu, Z.-L. Trends Food Sci. Tech.2025, 161, 105049.
[9] Chen L. J.; Jiang H. E.; Zheng M. H.; Li Z.; Li N.; Zhao S.; Liu, X. J. Hazard. Mater.2022, 434, 12222.
[10] Li, C. Q.; Zhang, W.; Xu, X. F.; Zhou, L. Journal of Agricultural and Food Chem 2025, 73, 4982-4997.
[11] Du W.; Gong X. L.; Tian Y.; Zhu X.; Peng Y.; Wang Y. W.Basel 2023, 13, 447.
[12] Miao X.; Wu C. X.; Li F.; Zhang, M. Adv. Funct. Mater2023, 33, 2212980.
[13] Zhang J.; Zhou J. J.; Zhang T. H.; Tang Y.; Zeng, L. Spectrochim. Acta A2023, 296,122647.
[14] Yang Y. X.; Yan X. M.; Liang T. Y.; Tian M.; Wu C.; Tang L.; Sun X.; Zhang J.; Li Y.; Zhong, K. J. Hazard. Mater2024, 469, 134003.
[15] Wang A. Y.; Tang F. Y.; Zang T.; Liu X.; Cheng G.; Chen W.; Shu W.; Li D.; Tang Y.; Sun X.; et al. Food Chem2024, 454, 139714.
[16] Cao R.; Zhang M. Y.; Tang W.; Wu J.; Wang M.; Niu X.; Liu Z.; Hao F.; Xu H.J. Fluoresc2023, 33, 1565-1576.
[17] Zhong K. L.; Hu L. N.; Du W. Q.; Sun X.; Tang L.; Yan X.; Li X.; Li J.Food Res. Int.2025, 201, 115613.
[18] Li Y.; Jiang X.; Li Y. Y.; Yan X.; Tang L.; Sun X.; Zhong K.; Li X.; Li J. Food Chem2024, 458, 140239.
[19] Singh S.; Velloth A.; Mahato R. R.; Grewal S.; Maiti S.; Venkataramani, S. Org. Biomol. Chem.2025, 23, 3634-3642.
[20] Zhang P.; Su J. L.; Zhen H.; Yu T.; Wei L.; Zheng M.; Zeng C.; Shu, W. Coord. Chem. Rev.2025, 522, 216232.
[21] Li Y. H.; Ma Y. l.; Huang T. R.; Huang J.; Shen K.; Li G.; Huang M. Microchem. J.2024, 204, 111018.
[22] Wang L. Y.; Ran X. G.; Tang H.; Cao D.Dyes Pigments2021, 194, 109634.
[23] Sun X. F.; Li J.; Gong, H. H. J. Chin. Inst. Food Sci. Technol2024, 24, 327-335 (in Chinese).
(孙小飞,李娟,宫慧慧,中国食品学报,2024, 24, 327-335)
[24] Erdemir S.; Oguz M.; Malkondu S.Talanta 2025, 292, 127906.
[25] Zhang T. T.; Lai Y. B.; Lin, W. Y. Sci. Total Environ2022, 837, 155462.
[26] Deng T.; Zhao J.; Peng D. F.; He X.; Huang X.-a.; Lin C.; Zhu C.; Wang L.; Liu, F. Org. Biomol. Chem2022, 20, 5076-5085.
[27] Zhong K. L.; Li Y.; Hu X. L.; Li Y.; Tang L.; Sun X.; Li X.; Zhang J.; Meng Y.; Ma R. Food Chem.2024, 438, 137987.
[28] Zhong K. L.; Liu S.; Li Y. Y.; Tang L.; Sun X.; Li X.; Li J.J. Sci. Food Agric.2025, 105, 3951-3961.
[29] Zhang J.; Yue C. Y.; Ke Y. J.; Qu H.; Zeng L. Adv. Agrochem2023, 2, 127-141.
[30] Ye H.; Ke Y.; Li W.; Zhu B.; Jiang L.; Hu X.; Zeng L.Anal. Chim. Acta2023, 1254, 341125.
[31] Sun X. F.; Jiang X.; Wang Z. D.; Li Y.; Ren J.; Zhong K.; Li X.; Tang L.; Li, J. J. Hazard. Mater.2024, 479, 135701.

基于“OFF-ON”型荧光探针的智能一体化平台在三文鱼肉新鲜度监测中的应用

Application of an Intelligent Integrated Platform Based on “OFF-ON” Fluorescent Probe in Salmon Freshness Monitoring

PDF

Supporting Info.

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Aopei Peng, Yang Li, Yuanyuan Zhu, Guangwen Wu, Shuangxi Gu. Advances in Chemoselective Recognition of Amino Acids by Schiff Base Fluorescent Probes [J]. Chinese Journal of Organic Chemistry, 2025, 45(9): 3301-3313.
[2]	Zhaozhou Li, Yafang Xie, Jian Tao, Xuebing Wei, Lele Li, Yajuan Li, Jiamin Tang, Huawei Niu, Xiujin Chen, Hongli Gao, Fang Li, Huichun Yu, Yunxia Yuan, Shaobin Gu, Huaibin Kang, Xiaofei Sun, Guoyan Ren, Ying Wu. Construction of Cysteine Fluorescent Probes and Their Applications in Pathological Examination [J]. Chinese Journal of Organic Chemistry, 2025, 45(7): 2335-2349.
[3]	Qing Shen, Yu Zeng, Zhonghao Li, Xiying Cao, Yuting Guo, Yunyi Ye, Zhaoyang Wang. Research Progress on Reactive Small Molecule Fluorescent Probes Based on the Formation and Cleavage of Covalent Bond [J]. Chinese Journal of Organic Chemistry, 2025, 45(4): 1194-1205.
[4]	Wenbo Zhang, Siming Su, Peng Yang. Synthesis of A Novel Calix[3]carbazole Derivative and Its Recognition and Detection of 10-Hydroxycamptothecin [J]. Chinese Journal of Organic Chemistry, 2025, 45(4): 1395-1401.
[5]	Hanyue Xiang, Shaoyin Wei, Yuji Wang, Nao Xiao. Recent Advances of Fe(II) Fluorescence Probes Based on N-Oxide Structure [J]. Chinese Journal of Organic Chemistry, 2025, 45(4): 1137-1152.
[6]	Xiaohong Cheng, Renjie Zhang, Jun You, Wenbin Fu, Wenjie Shen, Yunqiang Luo, Song Wang. A Novel Catalytic Fluorescent Probe and Its Application in Copper Detection in Gas-Generating Agents [J]. Chinese Journal of Organic Chemistry, 2025, 45(11): 4195-4201.
[7]	Bingyan Chen, Jie Sun, Linghong Xiong, Xuewen He. Fluorescence Light-Up Detection and Imaging of Atypical Nucleic Acid Structures [J]. Chinese Journal of Organic Chemistry, 2025, 45(11): 4082-4107.
[8]	Chun He, Shuqi Liu, Fenmin Cheng, Yuanqiang Hao, Shu Chen, Peisheng Zhang, Rongjin Zeng. Synthesis of Two-Photon Dansyl-Based Fluorescent Probe with Dual-State Emission Properties for Hydrogen Sulfide Detection [J]. Chinese Journal of Organic Chemistry, 2024, 44(9): 2869-2875.
[9]	Yu Zeng, Zhonghao Li, Siwei Deng, Zujia Chen, Biyu Chen, Shiwei Yu, Qing Shen, Zhaoyang Wang. Research Progress on Preparation and Application of Cyanoethylenes [J]. Chinese Journal of Organic Chemistry, 2024, 44(9): 2722-2731.
[10]	Xiaolong Su, Jianpeng Li, Mengxin Liu, Li Zou, Desuo Yang, Haitao Feng. Synthesis of Tetraphenylethylene Based Amides for Detection of Copper(II) Ion with High Sensitivity and Selectivity [J]. Chinese Journal of Organic Chemistry, 2024, 44(8): 2581-2587.
[11]	Yiran Hu, Siliang Zhang, Haiyan Luo, Luyao Zhao, Xudong Guo, Shuangqing Wang, Rui Hu, Guoqiang Yang. Design and Application of a Novel Chalcone Derivative Fluorescent Probe for Aminopeptidase N [J]. Chinese Journal of Organic Chemistry, 2024, 44(7): 2257-2264.
[12]	Lin Liu, Lin Chen, Xiaoling Hu, Keli Zhong, Jinglin Zhang, Lijun Tang. Application and Cell Imaging of Turn-On Fluorescent Probe for Hydrogen Sulfide Based on Benzopyran in Food Samples [J]. Chinese Journal of Organic Chemistry, 2024, 44(6): 2027-2032.
[13]	Wenyan Zhang, Dan Wang, Renjie Luo, Huiling Liu. Research Progress of Near-Infrared Fluorescent Surgical Navigation Probes [J]. Chinese Journal of Organic Chemistry, 2024, 44(6): 1760-1776.
[14]	Feifan Li, Kang Yu, Chuanzhi Ni, Yuanyuan Zhu, Jie Zeng, Shuangxi Gu. Chiral Fluorescent Probes for Determination of Both Concentration and Enantiomeric Composition of Amino Acids [J]. Chinese Journal of Organic Chemistry, 2024, 44(6): 1862-1869.
[15]	Jidong Zhang, Yao Yang, Jie Zhang, Wei She. Detection of Zn(II) by Tetraphenylethyene Fluorescent Probe Based on Aggregation-Induced Emission (AIE)-Excited State Intramolecular Proton Transfer (ESIPT) Effect [J]. Chinese Journal of Organic Chemistry, 2024, 44(4): 1337-1342.