Nine novel N-(1-ethoxycarbonylalkyl)-N'-β-pyridylcarbonylthiourea compounds were synthesized. The structures of the target compounds were determined by IR, ¹HNMR, MS spectra and elemental analysis. The crystal structure of the target compound 7d was determined by X-ray diffraction analysis. Crystal data: monoclinic, space group: C₂, a＝1.6912(6) nm, b＝0.51978(19) nm, c＝1.9399(7) nm, Z＝4, V＝1.6962(11) nm³, D_c＝1.266 Mg/m³, α＝90.00°, β＝95.890(5)°, γ＝90.00°, F(000)＝688, R＝0. 0667, wR＝0.1709. Using an MTT assay, the inhibitory rate of the title compounds against the K562 cell proliferation was measured. The result of a preliminary bioassay shows that some of the target compounds possess antiproliferation effects on K562 cells.

Key words: N-β-pyridylcarbonylthiourea, crystal structure, K562 cell, α-L-amino-acid ester