Progress in Precolumn Derivatization for HPLC Analysis of Glycans

Abstract

Glycans of glycoconjugates have biological and pathological significance. It is important to define glycan structure and correlate such structure with function. High-performance liquid chromatography is a widely used tool of the on-going research to isolate and purify glycans after release for subsequent analysis. This article reviews precolumn derivatization methods of glycans currently used for HPLC analysis. Advantages and problems of individual methods are summarized, while the native glycan recovery methods are also discussed.

Key words: high performance liquid chromatography, glycan, derivatization

Cite this article

WANG Zhong-Fu,HE Jian-Yu²,WEI Ya-Hui,HUANG Lin-Juan*^,1. Progress in Precolumn Derivatization for HPLC Analysis of Glycans[J]. Chin. J. Org. Chem., 2006, 26(05): 592-598.

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用于寡糖链分析的HPLC柱前衍生化方法研究进展