Application of mass spectrometry on the sequencing of peptides and proteins

Chin. J. Org. Chem. ›› 2002, Vol. 22 ›› Issue (2): 81-90. Previous Articles Next Articles

质谱在肽和蛋白质序列分析中的应用

陈晶;付华;陈益;赵玉芬

清华大学生命科学与工程研究院.北京(100084)

发布日期:2002-02-25

Application of mass spectrometry on the sequencing of peptides and proteins

Chen Jing;Fu Hua;Chen Yi;Zhao Yufen

Departement of Chemistry and Chemical Engineering, Qinghua University.Beijing(100084)

Published:2002-02-25

Abstract

Efficient and automatic methods of sequencing peptides and proteins are always the most demanded techniques in biological research. As a promising tool on the elucidation of molecular structures, mass spectrometry has been used in the sequencing of peptides and proteins for several years. It has become more and more popular in the biological work. This paper reviews the different methods on this technique, which include the activation methods (collision-activated dissociation, postsource decay, insource decay, et al.), derivative influence and so on.

Key words: PEPTIDE;PROTEIN;MASS SPECTRA;IONS;ACTIVATION;SEQUENCE, DETERMINATION

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Cite this article

Chen Jing;Fu Hua;Chen Yi;Zhao Yufen. Application of mass spectrometry on the sequencing of peptides and proteins[J]. Chin. J. Org. Chem., 2002, 22(2): 81-90.

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质谱在肽和蛋白质序列分析中的应用

Application of mass spectrometry on the sequencing of peptides and proteins

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