Chinese Journal of Organic Chemistry >
A Lysosome-Targeted Far-Red to Near-Infrared Fluorescent Probe for Monitoring Viscosity Change During the Ferroptosis Process
Received date: 2022-03-21
Revised date: 2022-05-29
Online published: 2022-06-17
Supported by
Taizhou Science and Technology Project(2003gy14); Taizhou Science and Technology Project(21ywb80); Scientific Research Project of Taizhou University(2019PY021); National College Students Innovation and Entrepreneurship Training Program(202010350033)
Elucidating the intrinsic complex relationship between pathophysiological processes and lysosomal viscosity is a tremendous challenge. So far, the change of lysosomal viscosity during the ferroptosis process has not been reported. In this study, a novel far-red to near-infrared viscosity fluorescent probe (FNVP) was designed and synthesized based on boron dipyrromethene (BODIPY) dye. The probe exhibited excellent responsiveness to viscosity change (λex=600 nm, λem=680 nm). In the phosphate buffered saline (PBS)-glycerol system (1~219 cP), the emission intensity of probe FNVP at 680 nm had about a 50-times increase. Furthermore, the probe showed the advantages of high selectivity, remarkable photostability and negligible cytotoxicity. Probe FNVP was lysosome-targetable and could be used for monitoring lysosomal viscosity changes induced by nystatin. Finally, using confocal fluorescence microscopy, lysosome viscosity was imaged during the ferroptosis process at the cellular level. All those will be beneficial to further comprehending the biological effectiveness of ferroptosis.
Key words： viscosity; fluorescent probe; lysosome; near-infrared; ferroptosis
Yanqin Lai , Xue Chen , Fang Chen , Linchen Ni , Ting Wang , Ziping Zhu , Ju Man , Chunxiao Jiang , Zhenda Xie . A Lysosome-Targeted Far-Red to Near-Infrared Fluorescent Probe for Monitoring Viscosity Change During the Ferroptosis Process[J]. Chinese Journal of Organic Chemistry, 2022 , 42(9) : 2850 -2856 . DOI: 10.6023/cjoc202203039
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