Chinese Journal of Organic Chemistry >
Studies toward Chemical Synthesis of Homogeneously Glycosylated Interleukin-10
Received date: 2024-10-02
Revised date: 2024-11-07
Online published: 2024-12-20
Supported by
National Natural Science Foundation of China(92153301); National Natural Science Foundation of China(22321005)
Human cytokine interleukin-10 (IL-10) sequence contains a conserved N-glycosylation sequon, Asn-Lys-Ser, but the presence and functional significance of this glycosylation have not been previously studied. Here, the chemical synthesis and characterization of IL-10 and its glycosylated form were explored. Using solid-phase peptide synthesis, native chemical ligation, and metal-free desulfurization, the full-length IL-10 was assembled and the non-glycosylated protein was successfully obtained through optimizing the refolding conditions. Building on this synthetic route, an N-acetylglucosamine (N-GlcNAc) modification was introduced into the peptide segment via cassette strategy, and further elaborated into Asn116 N-mono- saccharide-modified IL-10. Mass spectrometry (MS) and circular dichroism analysis of the synthesized IL-10 variants have suggested that they have similar structures to that of the biologically expressed counterpart.
Weidong Dong , Haiyun Liu , Xinyu Li , Biao Wang , Bo Cheng , Suwei Dong . Studies toward Chemical Synthesis of Homogeneously Glycosylated Interleukin-10[J]. Chinese Journal of Organic Chemistry, 2025 , 45(3) : 951 -958 . DOI: 10.6023/cjoc202410001
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