Fish freshness is closely related to food safety and human health. Therefore, it is of great significance to develop a rapid, simple, non-destructive and intuitive fish freshness detection method. Herein, an “OFF-ON” fluorescent probe PTD was successfully synthesized by using thiophenealdehyde and 2,4-thiazolidinedione through Knoevenagel condensation. The probe had dual recognition capability of colorimetric and fluorescence for amines in solution, with high sensitivity (1.20 μM) and fast response speed (3 s). The prepared PTD-loaded indicator label exhibited dual-channel response to the salmon freshness, with the daylight color changing from orange to earth-yellow and the fluorescence shifting from bright orange to flesh pink. By utilizing smartphone software and the indicator label, an intelligent integrated detection platform was constructed, which could automatically output the total volatile basic nitrogen (TVB-N) value of salmon. Under room temperature condition, its accuracy and reliability were confirmed by the Chinese national standard method. This study is expected to provide new ideas for portable intelligent detection fields and offer a smart reading tool for assessing fish freshness to merchants, consume
Sun Xiaofei
,
Zhang Haiyuan
,
Li Juan
,
Ma Ji
,
Wang Ruoxi
,
Zhong Keli
,
Li Xuepeng
,
Liang Tianyu
,
Tang Lijun
,
Li Jianrong
. Application of an Intelligent Integrated Platform Based on “OFF-ON” Fluorescent Probe in Salmon Freshness Monitoring[J]. Chinese Journal of Organic Chemistry, 0
: 251017
.
DOI: 10.6023/cjoc202507024
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