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化学学报
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化学学报 ›› 1991, Vol. 49 ›› Issue (12): 1478-1482. 上一篇    下一篇

研究论文

McGhee和von Hippel模型的改进及其在反铂-多核苷酸作用体系 中的应用

董艳红;邵伟平;唐雯霞;戴安邦   

  1. 南京大学配位化学研究所.南京(210008)
  • 发布日期:1991-12-15

Modifed McGhee and von Hippel model and its application in the system of the interaction of transplatin with polynucleotide

Dong Yanhong;Shao Weiping;Tang Wenxia;Dai Anbang   

  1. Najing Univ, Inst Coordinat Chem.Nanjing(210008)
  • Published:1991-12-15

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用条件概率原理推导了处理小分子与大分子格子中n个残基结合,并且覆盖n+1个(n≥2)连续格子残基这一类型键合体系的方程式,并在反铂-polyN体系中作了应用。测得了结合常数、每个铂配合物所键合的核苷酸残基数以及交叉结合和非交叉结合的比值。

关键词: 多核苷酸, 铂络合物, 模型, 残基(生化), McGhee, vonHippel, 结合常数, 覆盖

The equation describing small molecule binding to the n lattice residues and covering n+1 consecutive lattice residues (n≥2) has been derived by using simple conditional probabilities, and has been applied to the systems of the interaction of trans-[Pt(NH~3)~2Cl~2] with polynucleotides. The binding constants and the binding site size of trans-Pt to polyG, polyA and polyC at pH=5 and 7, and the ratio of overlap-cross linking to nonoverlap-cross linking formed between trans-Pt and polyN have been obtained.

Key words: POLYNUCLEOTIDE, PLATINUM COMPLEX, MODELS, RESIDUE (BIOCHEMISTRY), MULCH

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