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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (19): 2197-2201. 上一篇    

研究论文

铁和锰对桑蚕丝蛋白构象转变的影响

周文1, 黄郁芳2, 邵正中1, 陈新*,1   

  1. (1复旦大学高分子科学系 教育部聚合物分子工程重点实验室 上海 200433)
    (2复旦大学材料科学系 国家微分析中心 上海 200433)
  • 投稿日期:2007-03-29 修回日期:2007-05-19 发布日期:2007-10-14
  • 通讯作者: 陈新

Effect of Fe and Mn on the Conformation Transition of Bombyx mori Silk Fibroin

ZHOU Wen1; HUANG Yu-Fang2; SHAO Zheng-Zhong1; CHEN Xin*,1   

  1. (1 The Key Laboratory of Molecular Engineering of Polymers of the Ministry of Education of China, Department of Macromolecular Science, Fudan University, Shanghai 200433)
    (2 National Microanalysis Center, Department of Material Science, Fudan University, Shanghai 200433)
  • Received:2007-03-29 Revised:2007-05-19 Published:2007-10-14
  • Contact: CHEN Xin

PDF

794

被引次数

43

采用电感耦合等离子体质谱和原子吸收光谱对桑蚕丝腺体和蚕茧丝中Fe和Mn的含量进行表征, 同时采用拉曼光谱研究Fe(III)和Mn(II)对高浓度再生桑蚕丝蛋白水溶液中丝蛋白构象的影响. 研究结果表明, 桑蚕丝纤维中Fe的含量高于其在丝腺体中的含量, 而Mn的含量则相反. 在丝腺体中, Fe的含量从丝腺体的后部到前部逐渐增加; 但Mn含量的变化趋势与Fe不同, 它在丝腺体中部的含量最低. Fe(III)能够诱导丝蛋白的构象由无规线团和/或螺旋结构向β-折叠转变, 但是Mn(II)对丝蛋白的构象没有明显的影响.

关键词: 金属元素, 再生桑蚕丝蛋白浓溶液, 拉曼光谱, 构象转变, 吐丝机理, 酪氨酸

Inductively coupled plasma mass spectroscopy (ICP-MS) and atomic adsorption spectroscopy (AAS) were applied to determine the Fe and Mn contents in both silk glands and cocoon silks of Bombyx mori silkworms. In the meantime, Raman spectroscopy was used to monitor the effect of Fe(III) and Mn(II) on the conformation changes of silk protein in the concentrated regenerated silk fibroin solutions. The results showed that the Fe content in cocoon silk was higher than that in the silk gland, but the Mn content was on the other way round. In addition, the Fe content was gradually increased from the posterior part to the ante-rior part of the silk gland. However, the Mn content did not exhibit the same tendency as Fe did but showed the lowest value in the middle part of the silk gland. Finally the Raman spectra indicated that Fe(III) was able to induce the conformation transition of silk fibroin from random coil and/or helix conformation to β-sheet but Mn(II) seemed no such an effect.

Key words: metal element, concentrated regenerated silk fibroin solution, Raman spectroscopy, conformation transition, spinning mechanism, tyrosine