表面增强拉曼光谱技术在microRNA检测中的研究进展

doi:10.6023/A21010017

化学学报 ›› 2021, Vol. 79 ›› Issue (6): 694-704.DOI: 10.6023/A21010017 上一篇下一篇

所属专题：分子探针、纳米生物学与生命分析化学

综述

表面增强拉曼光谱技术在microRNA检测中的研究进展

易荣楠^a, 吴燕^b^,^*()

a 湖南警察学院刑事科学技术系长沙 410138
b 长江师范学院化学化工学院重庆 408100

投稿日期:2021-01-19 发布日期:2021-03-30
通讯作者: 吴燕

作者简介:

易荣楠, 男, 1992年生, 主要从事拉曼光谱技术在毒物毒品中的分析检验、有机合成方法学等方面的研究. 作为核心成员参与国家自然科学基金项目2项, 发表SCI论文8篇.

吴燕副教授, 女, 1988年生, 主要研究方向为SERS技术及生物传感器的应用研究. 主持国家自然科学基金、重庆市科委项目、重庆市教委项目、长江师范学院青年人才成长支持计划项目、长江师范学院科研启动项目等6项. 作为骨干成员参与多项国家自然科学基金、重庆市自然科学基金等项目的研究. 近年来, 以第一作者与通讯作者发表SCI论文12篇, 授权发明专利1项, 发表教改论文2篇.

基金资助:
国家自然科学基金(21804011)

Research Progress on Surface-Enhanced Raman Spectroscopy Technique for the Detection of microRNA

Rongnan Yi^a, Yan Wu^b()

a Criminal Technology Department, Hunan Police Academy, Changsha 410138, China
b College of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China

Received:2021-01-19 Published:2021-03-30
Contact: Yan Wu
Supported by:
National Natural Science Foundation of China(21804011)

文章分享

摘要/Abstract

microRNA是一段长约为18~24个核苷酸的内源性非编码单链RNA. 最新研究发现: 许多疾病和肿瘤的发生与microRNA的表达水平息息相关, 且microRNA有望成为新型肿瘤标志物及癌症治疗的新目标. 因此, 发展高灵敏度、高特异性及简单快速的microRNA分析检测方法对于生物医学研究和癌症的早期诊断具有重要的意义. 表面增强拉曼光谱(SERS)技术由于具有灵敏度高、检测速度快、指纹识别、水干扰小等独特优势, 在癌症的早期诊断领域具有很大的应用价值. 作者综述了SERS技术在microRNA检测方面的最新研究进展, 分析了该技术在生物检测中亟待解决的关键问题和挑战, 并对其未来的发展前景进行了展望.

关键词: 表面增强拉曼光谱, microRNA, 肿瘤标志物

microRNA is an endogenous non-coding single-stranded RNA with a length of about 18~24 nucleotides. The latest research has found that the occurrence of many diseases and tumors is closely related to the level of microRNA expression, and microRNA is expected to become a new tumor marker and a new target for cancer treatment. Therefore, the development of high sensitivity, high specificity, and simple and rapid microRNA detection methods is greatly significant for biomedical research and early diagnosis of cancer. Surface-enhanced Raman spectroscopy (SERS) technique has great application value in the field of early cancer diagnosis due to its unique advantages such as high sensitivity, fast detection speed, fingerprint recognition, and low water interference. The latest research progress of SERS technique in microRNA detection is summarized in this review. Finally the main challenges of SERS technique in bioassay are discussed and the future development trend is proposed.

Key words: surface-enhanced Raman spectroscopy, microRNA, tumor marker

引用此文

易荣楠, 吴燕. 表面增强拉曼光谱技术在microRNA检测中的研究进展[J]. 化学学报, 2021, 79(6): 694-704.

Rongnan Yi, Yan Wu. Research Progress on Surface-Enhanced Raman Spectroscopy Technique for the Detection of microRNA[J]. Acta Chimica Sinica, 2021, 79(6): 694-704.

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图/表 14

图1. 基于协同校正SERS策略对癌症相关microRNA-182的检测原理图[17]

Figure 1. Schematic illustration of a synergistic calibrated SERS strategy for the detection of cancer-related microRNA-182[17]

图2. 基于Fe3O4@PAD/Pt与DSN技术的SERS生物传感器对microRNA-155检测原理图[19]

Figure 2. Schematic for SERS biosensor based on Fe3O4@PDA/Pt and DSN signal amplification for microRNA-155 detection[19]

图3. (A) Y型DNA驱动的双层核壳金纳米结构的双信号分子CD与SERS探针用于肿瘤细胞内microRNA的检测原理图[25]; (B) CuPc@HG@BN探针的构建与其用于诊疗一体化的示意图[26]

Figure 3. (A) Schematic representation of Y-DNA-driven construction of Au NP chiral C30S5S10 NSs with enhanced CD and SERS signals for the detection of microRNA in tumor cell[25]; (B) illustration of the synthesis of CuPc@HG@BN probe and the antitumor and SERS monitoring mechanism[26]

图4. 基于DNA介导的金-银蘑菇状SERS纳米探针用于目标DNA/RNA的高灵敏多元检测原理图[27]

Figure 4. Schematic of DNA-mediated gold-silver nano-mushrooms as SERS probes for ultrasensitive and multiplex DNA/miRNA detection[27]

图5. 原位热点组装AgNP二聚体SERS探针用于microRNA-21的检测原理图[33]

Figure 5. Schematic of the AgNP dimer probes and the protocol for microRNA-21 detection with the in situ hot-spot assembly strategy[33]

图6. 基于目标microRNA驱动的AuNR手性边对边二聚体的光学传感器对细胞内microRNA检测原理图[36]

Figure 6. Schematic of the optical sensor based on target microRNA-directed formation of AuNR chiral side-by-side dimers for the detection of intracellular microRNA[36]

图7. DNA介导合成SERS纳米探针对多重microRNA的检测原理图[40]

Figure 7. Schematic illustration of the DNA-mediated SERS probe for multiplex microRNA detection[40]

图8. 基于目标microRNA触发的级联放大与银离子诱导放大的SERS传感平台用于microRNA检测原理图[50]

Figure 8. Schematic of SERS sensing platform for microRNA detection via microRNA-triggered hybridization chain reaction and Ag+-mediated cascade amplification [50]

图9. 基于锁式探针的指数滚环扩增技术的SERS传感器对microRNA-155的检测原理图[52]

Figure 9. Schematic of SERS sensor for microRNA-155 detection based on padlock probe-based exponential rolling circle amplification strategy[52]

图10. 基于双模式成像与反转信号比的BSR探针用于细胞内microRNA的定量检测原理图[66]

Figure 10. Schematic of dual-mode imaging and reverse signal ratio quantification of microRNA-203 in cells with BSR probes[66]

图11. 基于SERS-微流控平台的双信号放大机制检测microRNA-21的原理图[68]

Figure 11. Schematic diagram of duple signal amplification mechanism based SERS-microfluidics platform for determination of microRNA-21[68]

图12. 基于CHA技术耦合SERS-侧向层析技术的传感策略用于microRNA-21检测的原理图[71]

Figure 12. Schematic illustrations of principle of the SERS-based lateral flow sensing strategy coupled with CHA for the detection of microRNA-21[71]

图13. (A) Au-UCNP四面体检测microRNA的原理图; (B)用于检测microRNA的组装四面体用的DNA骨架[74]

Figure 13. Schematic illustrations of (A) principle of Au-UCNP pyramids for microRNA detection and (B) the nucleic acid skeleton of pyramid used for microRNA detection[74]

图14. 基于Ag/TiO2 NWs的SERS“三明治”生物传感器对microRNA-21检测的原理图[78]

Figure 14. Schematic illustrations of principle of the SERS-based sandwich biosensor coupled with Ag/TiO2 NWs for the detection of microRNA-21[78]

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表面增强拉曼光谱技术在microRNA检测中的研究进展

Research Progress on Surface-Enhanced Raman Spectroscopy Technique for the Detection of microRNA

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