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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (24): 2959-2964. 上一篇    下一篇

研究论文

新型三齿多吡啶钴(II)、钌(II)配合物的合成、表征及其与DNA的作用研究

杨浩*, 陈文涛, 邱东方, 包晓玉, 行文茹, 刘珊珊   

  1. (南阳师范学院化学系 南阳 473061)
  • 投稿日期:2007-03-22 修回日期:2007-08-15 发布日期:2007-12-28
  • 通讯作者: 杨浩

Synthesis, Characterization and DNA-Binding Studies of New Tridentate Polypyridyl Cobalt(II) and Ruthenium(II) Complexes

YANG Hao*; CHEN Wen-Tao; QIU Dong-Fang; BAO Xiao-Yu; XING Wen-Ru; LIU Shan-Shan   

  1. (Department of Chemistry, Nanyang Normal University, Nanyang 473061)
  • Received:2007-03-22 Revised:2007-08-15 Published:2007-12-28
  • Contact: YANG Hao

PDF

774

被引次数

31

合成了两种新型三齿多吡啶钴(II)和钌(II)的混配配合物[Co(TolylTPy)(H2Bzimpy)]Cl2 [TolylTPy＝4'-对甲基苯 基-2,2':6',2''-三联吡啶, H2Bzimpy＝2,6-二(苯并咪唑-2)吡啶] (A)和Ru(TolylTPy)(Bzimpy) (B). 用元素分析, IR, 1H NMR等对它们进行了表征, 测定了配合物B的晶体结构, 用电子吸收光谱、荧光光谱等研究了配合物与小牛胸腺DNA(CTDNA)的相互作用及其对pBR322 DNA的断裂作用. 结果表明, 配合物A和B与CTDNA的作用属静电结合, 凝胶电泳实验说明配合物A在310 nm光辐射15 min, 可使超螺旋pBR322 DNA断裂为开环缺口型和线型DNA.

关键词: 三齿多吡啶配体, 钴(II)、钌(II)配合物, DNA, 电子吸收光谱, 荧光光谱

Two novel tridentate polypyridyl mixed-ligand complexes of cobalt(II) and ruthenium(II), [Co(TolylTPy)(H2Bzimpy)]Cl2 [TolylTPy＝4'-p-tolyl-2,2':6',2''-terpyridine, H2Bzimpy＝2,6-bis(benzimi- dazol-2-yl)pyridine] (A) and Ru(TolylTPy)(Bzimpy) (B) were synthesized and characterized by elemental analysis, IR and 1H NMR. The crystal structure of complex B was determined. The interactions between the complexes and calf thymus DNA have been investigated by absorption and fluorescence spectroscopies. The photoactivated cleavage of pBR322 DNA by the complexes was studied. The spectrophotometric studies suggested that both complexes A and B bind to DNA by electrostatic interactions. The agarose gel electrophoresis showed that complex A converted supercoiled pBR322 DNA to nicked and linear DNA when it was irradiated at 310 nm for 15 min.

Key words: tridentate polypyridyl ligand, cobalt(II), ruthenium(II) complex, DNA, absorption spectra, fluorescence spectra