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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (4): 307-312. 上一篇    下一篇

研究论文

溶菌酶晶体生长过程中的扩散传质问题

段 俐 康 琦* 李根培

  

  1. (中国科学院力学研究所 国家微重力实验室 北京 100190)

  • 投稿日期:2007-12-15 修回日期:2008-09-25 发布日期:2009-02-28
  • 通讯作者: 康琦

Diffusion Mass Transfer in Lysozyme Crystal Growth

Duan, Li Kang, Qi* Li, Genpei   

  1. (National Microgravity Laboratory, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190)
  • Received:2007-12-15 Revised:2008-09-25 Published:2009-02-28
  • Contact: Kang, Qi

PDF

1194

被引次数

7

采用Mach-Zehnder干涉测量系统与图象处理系统相结合实时观测溶菌酶蛋白质晶体生长过程中的流体动力学现象, 对汽相扩散法溶菌酶蛋白质晶体生长过程进行了研究, 观测到溶菌酶晶体生长时晶体周围存在的干涉条纹. 分析了晶体生长时的重力沉降效应和溶质扩散过程. 实验结果表明, 该生长过程中的流体物理特征直接影响了生长晶体的晶面. 根据实验结果, 给出对蛋白质晶体生长过程流体物理现象的讨论.

关键词: 干涉测量, 晶体生长, 扩散, 重力沉降

An optical diagnostic system consisting of a Mach-Zehnder interferometer with an image processor was used to study the kinetic process of crystal growth. The crystallization process of lysozyme protein by vapor diffusion was investigated. The interference fringes around the growing lysozyme crystal were observed in real time. The effects of sedimentation and diffusion were analyzed. The experiment demonstrates that the fluid physics characteristic has an obvious influence on the solidification of the crystal growing face. According to the present experiment, a discussion on the protein crystal growth has been advanced.

Key words: interference measurement, crystal growth, diffusion, sedimentation