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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (02): 199-203. 上一篇    下一篇

研究论文

纳米粒子标记激光诱导荧光免疫分析测定癌胚抗原

张晓明，章竹君*，薛盼   

  1. (陕西师范大学化学与材料科学学院 陕西省生命分析重点实验室 西安 710062)
  • 投稿日期:2010-06-21 修回日期:2010-08-13 发布日期:2010-09-30
  • 通讯作者: 章竹君 E-mail:zxm-205@163.com

Immunoassay for Carcinoembryonic Antigen Based on Fluorescence Induced by Laser and Labeled with Nanoparticles

ZHANG Xiao-Ming, ZHANG Zhu-Jun, XUE Pan   

  1. (School of Chemistry and Materials Science, Shaanxi Normal University, Key Laboratory of Life Analysis of Shaanxi Province, Xi an 710062)
  • Received:2010-06-21 Revised:2010-08-13 Published:2010-09-30
  • Contact: Zhu-Jun ZHANG E-mail:zxm-205@163.com

PDF

1246

被引次数

6

建立了一种核壳型SiO2荧光纳米粒子标记的激光诱导荧光免疫测定的新方法. 该方法用 掺杂的核壳型SiO2荧光纳米粒子为标记物, 采用双抗体夹心法, 在载玻片上固定鼠抗人癌胚抗原(CEA)单克隆抗体, 加入1～2 μL样品, 再加入纳米粒子标记的另一种鼠抗人癌胚抗原(CEA)单克隆抗体. 识别后在光导纤维激光诱导荧光毫米阵列检测平台上测量荧光信号, 进行定量. 在优化条件下, 荧光强度和癌胚抗原的量在1～80 pg的范围内具有良好的线性关系, 相关系数r＝0.9933, 方法的检出限为0.3 pg (20 amol). 对40 pg的CEA平行测定5次, 相对标准偏差为5.5%.

关键词: 纳米粒子, 激光诱导荧光, 阵列传感器, 癌胚抗原

A new immunoassay method for Carcinoembryonic Antigen based on fluorescence induced by laser and labeled with nanoparticles is presented. Core-shell SiO2 fluorescent nanoparticles were used as labels, “sandwich” model was used. Mouse anti-carcinoembryonic antigen (CEA) monoclonal antibody was immobilized on slides, then 1～2 µL CEA was added, and the other mouse anti-CEA monoclonal antibody labeled with nanoparticles was added, after CEA and antibody recognized, the fluorescent signal was quantified with an laser-induced fluorescence millimeter array detection platform based on optical fiber. The fluorescence intensity has a linear relationship with the concentration of CEA in the range of 1～80 pg, with the absolute limit detection of CEA being 0.3 pg. The relative standard deviation (RSD) for 5 parallel measurements of CEA (40 pg) was 5.5%.

Key words: nanoparticles, laser induced fluorescence, sensor array, carcinoembryonic antigen

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