化学学报 ›› 2002, Vol. 60 ›› Issue (9): 1727-1731. 上一篇    下一篇

研究论文

基于美洲蜚蠊下颚须的整合受体生物传感器测定抗坏血酸

洪丽;尹屹梅;郑赛晶;段亚楠;林祥钦   

  1. 中国科学技术大学化学系,合肥(230026)
  • 出版日期:2002-09-15 发布日期:2002-09-15

Intact-Chemoreceptor-Based Biosensor: Detection of Ascorbic Acid

Hong Li;Yin Yimei;Zheng Saijing;Duan Yanan;Lin Xiangqin   

  1. Department of Chemistry, University of Science and Technology of China,Hefei(230026)
  • Online:2002-09-15 Published:2002-09-15

报道了以美洲蜚蠊的下颚须作为分子识别和转换元件研制的一种新型生物组织 传感器,发现该传感器对抗坏血酸(AA)有选择性的响应,响应脉冲的发放频率与 AA的浓度对数成正比,在5 * 10~(-5)至5 * 10~(-2)mol/L范围内呈线性关系,检 测下限低于10~(-6)mol/L AA。该传感器响应快,并具有较好的重现性。

关键词: 生物传感器, 维生素C

An intact-chemoreceptor-based biosensor based on the gustatory organ, the maxillary palp of Periplaneta americana, is reported, in which the maxillary palp serves as not only the molecular recognition elements but also the biological transducers. The binding events between ligand and chemoreceptor induce action potentials (APs) whose frequency correlates directly to the concentration of stimulants. The APs were recorded with a three-electrode system and collected by sound card method developed by our group. This is the first time that the biosensor is reported to yield quantitative linear dose-response data over the concentration range from 5 * 10~(-5) to 5 * 10~(-2) mol/L. The new biosensor possesses some advantages over the biosensor based on isolated receptor: shorter response time (<1 s), higher degrees of sensitivity (detect-limitation below 10~(-6) mol/L), broader dynamic response range (over four orders of magnitude) and more preferable reproducibility.

Key words: BIOSENSORS, VITAMINC

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