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化学学报
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化学学报 ›› 2007, Vol. 65 ›› Issue (13): 1239-1242. 上一篇    下一篇

研究论文

一种简便灵敏测定C反应蛋白的免疫共振散射光谱分析新方法

梁爱惠*, 张南南   

  1. (桂林工学院材料与化学工程系 有色金属与材料加工新技术教育部重点实验室 桂林 541004)
  • 投稿日期:2006-11-29 修回日期:2007-01-30 发布日期:2007-07-14
  • 通讯作者: 梁爱惠

A Simple and Sensitive Immunoresonance Scattering Spectral Assay for C Reactive Protein

LIANG Ai-Hui*; ZHANG Nan-Nan   

  1. (Key Laboratory of New Processing Technology for Nonferrous Metals and Materials of Ministry of Edu-cation, Department of Materials and Chemical Engineering, Guilin University of Technology, Guilin 541004)
  • Received:2006-11-29 Revised:2007-01-30 Published:2007-07-14
  • Contact: LIANG Ai-Hui

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854

被引次数

16 | 3

在pH 6.0的柠檬酸-Na2HPO4缓冲溶液中及PEG-6000存在下, C反应蛋白(CRP)与羊抗人C反应蛋白可聚集形成免疫复合物微粒, 在350, 390, 440 nm处有三个共振散射峰. 激光散射法测得免疫复合物微粒的平均粒径为1720.0 nm. 在最佳实验条件下, CRP浓度在0.03～1.80 μg•mL－1的范围内与390, 440 nm处共振散射强度都呈良好的线性关系, 其回归方程、相关系数、检出限分别为ΔI390 nm＝306.4c＋17.3, ΔI440 nm＝296.0c＋10.7; 0.9993, 0.9996; 0.011, 0.012 μg•mL－1. 该方法选择性较好, 操作简便, 用于人血清中C反应蛋白的测定, 结果与免疫透射比浊法结果一致, 相对标准偏差在0.90%～4.12%.

关键词: C反应蛋白, 免疫复合物微粒, 免疫反应, 共振散射

In pH 6.0 citric acid-Na2HPO4 buffer solution goat-anti-human C reactive protein (CRP) was combinated with human CRP specifically, to aggregate with formation of immune complex particles that exhibited three resonance scattering peaks at 350, 390 and 440 nm respectively, in the presence of PEG-6000. The laser scattering measurement indicates that the average diameter of the immune complex particles is 1720.0 nm. CRP concentration in the range from 0.03 to 1.80 μg•mL－1 is all proportional to the resonance scattering intensity at 390 and 440 nm. Its regression equation is ΔI390 nm＝306.4c＋17.3 and ΔI440 nm＝296.0c＋10.7, the correlation coefficient is 0.9993 and 0.9996, and the detection limit is 0.011 and 0.012 μg•mL－1, respectively. The method has been applied to the determination of CRP in the human blood serum, and its results are in agreement with those of the immunoturbity, with relative standard deviation of 0.90%～4.12%.

Key words: C reactive protein, immune complex particle, immunoreaction, resonance scattering