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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (06): 535-539. 上一篇    下一篇

研究论文

Tyr/Glu/Fe3O4/Nafion/CNT/GCE酪氨酸酶生物传感器的制备及应用于农药检测的研究

曲云鹤，肖飞，程欲晓，施国跃，金利通   

  1. (华东师范大学化学系 上海 200062)
  • 投稿日期:2009-09-09 修回日期:2009-10-15 发布日期:2010-03-28
  • 通讯作者: 金利通 E-mail:ltjin@chem.ecnu.edu.cn,litongjin@263.net,51050606028@student.ecnu.edu.cn
  • 基金资助:

    上海世博专项（06dz05824）

Construction of Tyr/Glu/Fe3O4/Nafion/CNT/GCE Biosensor for Detection of Pesticide

Qu Yunhe Xiao Fei Cheng Yuxiao Shi Guoyue Jin Litong   

  1. (Department of Chemistry, East China Normal University, Shanghai 200062)
  • Received:2009-09-09 Revised:2009-10-15 Published:2010-03-28

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制备了磁性四氧化三铁纳米粒子, 采用碳纳米管、Nafion等功能性材料构建了酪氨酸酶(Tyr)生物传感器, 并将其应用于农药的检测. 实验表明, 四氧化三铁纳米粒子具有良好的生物兼容性, 碳纳米管能够有效地促进电子传递, 修饰了四氧化三铁纳米粒子等功能性材料的酶传感器, 响应速度快, 检测灵敏度高, 稳定性好; 固定在传感器上的酪氨酸酶有良好的酶动力学响应, 其表观米氏常数为61.5 μmol/L. 利用农药对酪氨酸酶的抑制作用, 以苯酚为底物, 对农药呋喃丹进行了检测, 检测限达到2.0×10－9 mol/L.

关键词: 四氧化三铁, 酪氨酸酶, 农药, 呋喃丹

A Tyr/Glu/Fe3O4/Nafion/CNT multilayer modified electrode was employed for the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provides a favorable environment for the Tyr electrocatalytic characterization and fast electron transfer. The surface of the sensor was characterized by TEM methods. A set of experimental conditions were also optimized for the detection of the pesticide. The developed biosensor showed high sensitivity, stability and reproducibility, which had a low detection limit of 2.0×10－9 mol/L for carbofuran.

Key words: Fe3O4, Tyr, pesticide, carbofuran