乌头与贝母配伍化学成分变化的UPLC/Q-TOFMS研究

化学学报 ›› 2011, Vol. 69 ›› Issue (16): 1920-1928. 上一篇下一篇

研究论文

乌头与贝母配伍化学成分变化的UPLC/Q-TOFMS研究

王超^1,2，王宇光*^,2，梁乾德²，让蔚清¹，肖成荣²，高月*^,2

(¹南华大学生命科学研究中心环境医学与放射卫生研究所衡阳 421001)
(²军事医学科学院放射与辐射医学研究所药理与毒理研究室北京 100850)

投稿日期:2010-10-23 修回日期:2011-01-18 发布日期:2011-04-08
通讯作者: 王超 E-mail:joebiz@163.com
基金资助:
973中医理论专项;国家自然基金资助项目

Analysis of Chemical Composition in Combination of Aconitum and Fritillaria by UPLC/Q-TOFMS with Multivariate Statistical Analysis

Wang Chao^1,2 Wang Yuguang*^,2 Liang Qiande² Rang Weiqing¹ Xiao Chengrong² Gao Yue*^,2

(¹ Institute of Environmental Medicine and Radiation Hygiene, Research Center of Life Science, University of South China, Hengyang 421001)
(² Institute of Radiation and Irradiation Medicine, Academy of Military Medical Sciences, Beijing 100850)

Received:2010-10-23 Revised:2011-01-18 Published:2011-04-08

文章分享

摘要/Abstract

利用UPLC/Q-TOFMS对中药十八反药对——乌头贝母配伍的合煎液与合并液进行检测, 经过MassLynx 4.1分析其化学成分存在差异, 发现合煎后次乌头碱水解受到部分抑制, 而其他主要的双酯型二萜生物碱溶出较少且水解较彻底, 乌头与川贝配伍合煎的毒性相对较小, 因而可能通过合理炮制减毒后应用于临床治疗.

关键词: 十八反, UPLC, Q-TOFMS, 液质联用, 乌头, 贝母

An ultra performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC/Q-TOFMS) based chemical profiling approach to evaluate chemical constitution between mixed decoction and co-decoction of Radix aconiti-Fritillaria combination of the Eighteen Incompatible Medications (Shi Ba Fan) was proposed. Two different kinds of decoctions, namely Radix aconiti-Fritillaria co- decoction: water extract of mixed two herbs, and Radix aconiti-Fritillaria mixed decoction: mixed water extract of each individual herbs, were prepared. Batches of these two kinds of decoction samples were subjected to UPLC/Q-TOFMS analysis, the datasets were processed with MassLynx 4.1 to holistically compare the difference between these two kinds of decoction samples. The most changed components during decocting were analyzed. Using the proposed approach, global chemical difference was found between co-decoction and mixed decoction, hypaconitine was identified as the most changed toxic components (changed most significantly) during co-decocting. Result shows the hydrolysis of hypaconitine were inhibited after co-decoction of Radix aconiti-Fritillaria, and other major diester diterpenoid alkaloids were hydrolysis most completely. For the co-decoction of Radix aconite combined with Bulbus fritillariae cirrhosae, the content of hypaconitine and deoxyaconitine is more than that of Radix aconiti-Bulbus fritillariae thunbergii.

Key words: eighteen incompatible medications, UPLC, Q-TOFMS, LC-MS, Aconitum, Fritillaria

引用此文

王超, 王宇光, 梁乾德, 让蔚清, 肖成荣, 高月. 乌头与贝母配伍化学成分变化的UPLC/Q-TOFMS研究[J]. 化学学报, 2011, 69(16): 1920-1928.

WANG Chao, WANG Yu-Guang, LIANG Qian-De, RANG Wei-Qing, XIAO Cheng-Rong, GAO Yue. Analysis of Chemical Composition in Combination of Aconitum and Fritillaria by UPLC/Q-TOFMS with Multivariate Statistical Analysis[J]. Acta Chimica Sinica, 2011, 69(16): 1920-1928.

导出引用 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

[1]	陈玉宛, 周雯, 李欣蔚, 杨开广, 梁振, 张丽华, 张玉奎. 基于液质联用技术的蛋白质-蛋白质相互作用研究进展^※[J]. 化学学报, 2022, 80(6): 817-826.
[2]	李飞, 王宇光, 杨亮, 朱冠秀, 梁乾德, 马增春, 肖成荣, 谭洪玲, 汤响林, 高月. UPLC-Q-TOF/MS分析不同比例丹参配伍藜芦毒性生物碱变化规律[J]. 化学学报, 2012, 70(21): 2257-2264.
[3]	朱粉霞, 赵永刚, 贾晓斌, 韦英杰, 张振海, 古丽娜尔·吾拉孜汗. 淫羊藿炮制前后UPLC-PDA-MS 的指纹图谱研究[J]. 化学学报, 2012, 70(05): 635-642 .
[4]	辛杨, 皮子凤, 宋凤瑞, 刘志强, 刘淑莹. 利用液质联用技术分析干姜对乌头类生物碱在大鼠肠内吸收的影响[J]. 化学学报, 2012, 70(05): 551-560 .
[5]	周思思, 马增春, 梁乾德, 王宇光, 谭洪玲, 肖成荣, 张伯礼, 高月. 基于UPLC/Q-TOF-MS 分析附子半夏配伍相反的物质基础[J]. 化学学报, 2012, 70(03): 284-290.
[6]	刘璐莎, 樊君, 胡春梅, 孙洋, 胡晓云, 赵英永, 魏嵩, 梁旭华. 土贝母皂苷II与人血清白蛋白相互作用机制的光谱研究[J]. 化学学报, 2011, 69(21): 2589-2596.
[7]	韩天娇, 宋凤瑞, 刘忠英, 皮子凤, 刘志强, 刘淑莹. 附子配伍过程中二萜类生物碱在Caco-2小肠吸收细胞模型中吸收转运的UPLC/MS研究[J]. 化学学报, 2011, 69(15): 1795-1802.
[8]	刘文龙, 宋凤瑞, 刘志强, 刘淑莹, 张大方. 川乌与半夏、瓜蒌、贝母、白蔹、白芨配伍禁忌的化学研究[J]. 化学学报, 2010, 68(9): 889-896.
[9]	随志刚, 姜雅秋, 刘志强, 梁峰, 闫峻, 刘忠英. 乌头碱在家兔肠道内代谢产物的LC/ESI-MSⁿ研究[J]. 化学学报, 2009, 67(21): 2439-2444.
[10]	赵宇峰,宋凤瑞,王曦烨,国新华,刘志强,刘淑莹. 16-O-去甲基乌头碱的生物转化及电喷雾质谱研究[J]. 化学学报, 2008, 66(5): 525-530.
[11]	周莉莉,刘志强,吴国光,宋凤瑞,刘淑莹. 山茱萸炮制过程中环烯醚萜苷类成分的质谱研究[J]. 化学学报, 2008, 66(24): 2712-2716.
[12]	越皓,皮子凤,宋凤瑞,刘志强,刘淑莹^{. 生附片化学成分的HPLC/ESI-MSⁿ研究[J]. 化学学报, 2008, 66(2): 211-215.}
[13]	张旭, 宋凤瑞, 王隶书, 刘志强, 刘淑莹^{. 人参与藜芦配伍化学成分变化的HPLC-ESI-MS与ESI-MS研究[J]. 化学学报, 2007, 65(9): 829-833.}
[14]	陈东林,简锡贤,陈巧鸿,王锋鹏. 直缘乌头中新的C19—二萜生物碱的结构研究[J]. 化学学报, 2003, 61(6): 901-906.
[15]	王锋鹏,李正邦,王建忠,简锡贤,陈东林. 膝瓣乌头根中新去甲二萜生物碱的结构研究[J]. 化学学报, 2000, 58(5): 576-579.

乌头与贝母配伍化学成分变化的UPLC/Q-TOFMS研究

Analysis of Chemical Composition in Combination of Aconitum and Fritillaria by UPLC/Q-TOFMS with Multivariate Statistical Analysis

PDF

可视化

被引次数

摘要/Abstract

引用此文

分享此文

参考文献

相关文章 15

编辑推荐

相关统计

本文评价