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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (20): 2486-2492. 上一篇    下一篇

研究论文

生物亲和性壳聚糖-二氧化硅复合纳米粒子的合成及其吸附DNA特性研究

张李娜1，郭志慧1，郑行望*,1，汪绒1，孟美荣1，屈颖娟1，刘全宏2，姚莎1   

  1. (1陕西省生命分析化学重点实验室 陕西师范大学化学与材料科学学院 西安 710062)
    (2陕西师范大学生命科学学院 西安 710062)
  • 投稿日期:2011-03-21 修回日期:2011-06-22 发布日期:2011-06-30
  • 通讯作者: 郑行望 E-mail:zhengxw@snnu.edu.cn
  • 基金资助:

    国家自然科学基金项目;国家文物局项目

Synthesis of Biocompatible Chitosan-doped SiO2 Nanoparticles and Its Application for DNA Adsorption

Zhang Lina1 Guo Zhihui1 Zheng Xingwang*,1 Wang Rong1 Meng Meirong1 Qu Yingjuan1 Liu Quanhong2 Yao Sha1   

  1. (1 Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Xi′an 710062)
    (2 College of Life Sciences, Shaanxi Normal University, Xi′an 710062)
  • Received:2011-03-21 Revised:2011-06-22 Published:2011-06-30
  • Contact: ZHENG Xing-Wang E-mail:zhengxw@snnu.edu.cn

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1799

被引次数

21

采用反相微乳液法制备了壳聚糖-二氧化硅复合纳米粒子, 通过透射电镜(TEM)、红外光谱方法、Zeta电位实验等对所合成的复合纳米粒子的结构和性质等进行了表征. 结果显示, 基于壳聚糖与silica之间超分子作用所形成的复合纳米粒子呈规则的球形, 粒径为50 nm左右, 具有良好的单分散性和微孔结构, Zeta电位测定结果表明复合纳米粒子具有正的ζ (Zeta)电位值. 基于复合纳米粒子的微孔结构和显正电性, 以小牛胸腺DNA为对象研究了复合纳米粒子对DNA的吸附特性. 研究结果表明在pH＝6的磷酸盐(PBS)缓冲中, 复合纳米粒子不但呈现出对小牛胸腺DNA良好的吸附能力, 而且还由于壳聚糖与SiO2纳米材料的复合而展现出优良的抗酶切割能力和细胞亲和性. 据此发现, 我们发展了一种有望用于向细胞内转载DNA的新材料.

关键词: 壳聚糖-二氧化硅复合纳米粒子, 反相微乳液法, 小牛胸腺DNA

A novel mesoporous chitosan-silica nanoparticles was synthesized with the water-in-oil (W/O) reverse microemulsion method. By characterizing this nanoparticles with transmission electron microscopy, IR spectra and the zeta potential experiments, it was found that those nanoparticles were spherical and uniform with 50 nm size and presented the mesoporous structure. Based on these findings, the adsorption ability of this nanoparticles for calf thymus DNA was studied. The relating results showed that this nanoparticles had a stronger adsorption ability to DNA in PBS buffer (pH＝6), they also presented the better biocompatibility for cell and could be used for protect DNA from Dnase I cleavage.

Key words: chitosan-silica nanoparticles, reverse microemulsion, calf thymus DNA