Abstract：Silver doped hydroxyapatite (HA) antimicrobial powders were synthesized by hydrothermal method using Ca(NO3)2?4H2O, AgNO3, (NH4)2HPO4 as raw materials. The effects of addition of AgNO3, reactive temperature and time on the microstructure and constituent of Ag-HA were investigated. The XRD patterns indicate that HA and Ag-HA have the same crystal structure. The calculation results of energy dispersive spectrometer (EDS) and X-ray fluorescence spectrum (XRF) indicate that Ca2+ in HA crystals is displaced by Ag+ and AgxCa10－x(PO4)6(OH)2 was produced at hydrothermal condition. The antibacterial test results indicate that Ag-HA have favourable antibiotic property and MIC≤50 μg/mL against Escherichia coli and Staphylococcus aureus.