化学学报 ›› 2012, Vol. 70 ›› Issue (19): 2005-2011.DOI: 10.6023/A12060339 上一篇    下一篇

综述

核磁共振波谱在分析化学领域应用的新进展

王桂芳a,b, 马廷灿a,b, 刘买利c   

  1. a 中国科学院国家科学图书馆武汉分馆 武汉 430071;
    b 中国科学院武汉文献情报中心 武汉 430071;
    c 中国科学院武汉物理与数学研究所 波谱与原子分子物理国家重点实验室 武汉磁共振中心 武汉 430071
  • 收稿日期:2012-06-25 出版日期:2012-10-14 发布日期:2012-08-10
  • 通讯作者: 刘买利 E-mail:ml.liu@wipm.ac.cn
  • 基金资助:

    项目受“重大新药创新”科技重大专项(No. 2010ZX09401-305-07)和国家自然科学基金(No. 21175127)资助.

Recent Progress in the Nuclear Magnetic Resonance Applications in Analytical Chemistry

Wang Guifanga,b, Ma Tingcana,b, Liu Mailic   

  1. a Wuhan Branch of the National Science Library, Chinese Academy of Sciences, Wuhan 430071, China;
    b Wuhan Documentation and Information Center, Chinese Academy of Sciences, Wuhan 430071, China;
    c State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan 430071, China
  • Received:2012-06-25 Online:2012-10-14 Published:2012-08-10
  • Supported by:

    Project supported by the Major Special Project of Scientific and Technological: Major Creation of New Drugs (No. 2010ZX09401-305-07) and the National Natural Science Foundation of China (No. 21175127).

发展检测物质的化学组成、结构及其变化的新方法、新技术是分析化学的核心科学问题之一. 波谱分析(光谱、质谱及核磁共振)是分析化学中常用的主要仪器分析手段. 核磁共振能在液态、固态和气态条件下提供复杂体系中分子组成、原子分辨的三维结构、相互作用和动态过程等丰富信息, 在生物分析中发挥着越来越重要的作用. 本文将综合阐述核磁共振技术在生物大分子体系、复杂体系以及与其他分析手段联用的进展情况.

关键词: 核磁共振, 分析化学, 复杂体系, 生物大分子

Development of new and effective methods for measuring chemical composition, molecular structures, interactions and dynamics is one of the major issues of analytical chemistry. Spectral analysis (spectroscopy, mass spectrometry and nuclear magnetic resonance) is the most commonly used analytical tool to address these issues. Nuclear magnetic resonance is capable to determine structure for small molecules, macromolecules and complicated biological systems, and it is considered as the most powerful tool in analytical chemistry. This paper reviewed recent progress of nuclear magnetic resonance in biological macromolecules system, complex system and the hyphenated method applications in analytical chemistry. In the first part, we gave a brief introduction of nuclear magnetic resonance technology and its applications in analytical chemistry field. The detailed application descriptions of nuclear magnetic resonance technology have been summarized from part two to part four. In the second part, we summarized the applications of nuclear magnetic resonance technology in biological macromolecules system, including the main nuclear magnetic resonance technology development in three dimensional protein structural analysis field and its applications; the related methods and applications in the dynamic study of protein complex, the in-cell nuclear magnetic resonance labeling methods development history and its applications, and also the methods of nuclear magnetic resonance technologies in studying the interactions of protein and drugs. In the third part, the qualitative and quantitative analysis of nuclear magnetic resonance technologies in complex systems has been summarized, including the applications in the metabolomics and the applications in the field of food quality and safety. In the fourth part, we briefly introduced the joint applications of magnetic resonance technologies and other separation methods such as chromatography and spectroscopic ways. The conclusions of nuclear magnetic resonance technology applications and the suggestions of future developing directions were stated in the last part.

Key words: nuclear magnetic resonance, analytical chemistry, complex system, biomacromolecules