Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (23): 2865-2869.DOI: 10.6023/A1012104 Previous Articles     Next Articles

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陈昌云*,1, 张红琳1, 柳闽生1, 颜妍2, 赵波2   

  1. (1 南京晓庄学院生物化工与环境工程学院 南京 211171)
    (2 南京师范大学化学与材料科学学院 南京 210097)
  • 投稿日期:2010-12-10 修回日期:2011-02-10 发布日期:2011-08-15
  • 通讯作者: 陈昌云
  • 基金资助:


An Electrochemical Immunosensor for the Determination of Ractopamine Based on Multi-walled Carbon Nanotubes and Ionic Liquid Composite

Chen Changyun*,1; Zhang Honglin1; Liu Minsheng1; Yan Yan2; Zhao Bo2   

  1. (1 School of Biochemical & Environmental Engineering, Nanjing Xiaozhuang University, Nanjing 211171)
    (2 College of Chemistry and Material Science, Nanjing Normal University, Nanjing 210097)
  • Received:2010-12-10 Revised:2011-02-10 Published:2011-08-15

We have developed a new method of electrochemical immunosensor for the determination of ractopamine (RAC). The modified electrodes were prepared as follows. First, the multi-walled carbon nanotubes and ionic liquid composites were cast on glassy carbon electrode (GCE), and then the ractopamine-bovine serum albumin antigens were cast on GCE. At last, we dipped a few Nafion onto the electrode. Cyclic voltammetry and differential pulse voltammetry were employed to monitor the fabrication steps of immunoreaction system in solutions containing K3Fe(CN)6 as redox probes. The immunosensor demonstrates excellent RAC detection features with a broad linear range of 1 to 1500 ng/mL and a detection limit of 0.3 ng/mL. The tests with spiked animal feed samples showed excellent recovery for ractopamine.

Key words: multi-walled carbon nanotube, ionic liquid, ractopamine, electrochemical immunosensor