Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (13): 1483-1487.

Article

### 纳米金颗粒增强信号的表面等离子体共振生物传感器用于甲氧檗因高灵敏检测的研究

1. 湖南大学化学生物传感与计量学国家重点实验室 化学化工学院 生物纳米与分子工程湖南省重点实验室 长沙 410082
• 投稿日期:2012-04-10 发布日期:2012-05-26
• 通讯作者: 羊小海, 王柯敏 E-mail:yangxiaohai@hnu.edu.cn; kmwang@hnu.edu.cn
• 基金资助:

项目受国家自然科学基金(Nos. 21190044, 21175035)、973计划(No. 2011CB911002)、科技部国际合作重大项目(No. 2010DFB30300)、教育部“新世纪优秀人才支持计划”(No. NCET-09-0338)和湖南省高等学校青年骨干教师培养对象基金(2009年度)资助.

### High Sensitive Coralyne Detection by Using of Au Nanoparticles-Enhanced Surface Plasmon Resonance Biosensor

Wang Qing, Zhu Hongzhi, Yang Xiaohai, Wang Kemin, Yang Lijuan, Ding Jing

1. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key La-boratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082
• Received:2012-04-10 Published:2012-05-26
• Supported by:

Project supported by the National Natural Science Foundation of China (Nos. 21190044, 21175035), National Basic Research Program (No. 2011CB911002), International Science & Technology Cooperation Program of China (No. 2010DFB30300), and Program for New Century Excellent Talents in University (No. NCET-09-0338), Hunan Province Ordinary College Young Teachers Training Project (2009).

Coralyne, a kind of planar alkaloid, has more pronounced antitumor activity compared to other protoberberine alkaloids. It was found that coralyne could induce the adenine (A)-rich DNA oligonucleotides to form a double strand DNA, with a stoichiometry of one coralyne per four adenine bases. In this paper, a high sensitive approach for the detection of anticancer drug coralyne based on surface plasmon resonance (SPR) and the high selectivity and sensitivity recognition process of coralyne with the A-rich DNA oligonucleotides was established. Generally, it was difficult to detect small molecules directly using conventional SPR biosensors since the changes of refractive index, which was resulted by binding small molecules, were usually small. Here, the Au nanoparticles was introduced for enhancing the sensitivity. The A-rich DNA strands were modified on Au nanoparticles and on Au film respectively. In the presence of coralyne and the functional Au nanoparticles, the A-rich DNA strands could form the duplex through the specific coordination between coralyne and adenine, and then the functional Au nanoparticles were captured on the Au film. SPR signal was enhanced by the electronic coupling between the localized plasmon of Au nanoparticles and the surface plasmon wave associated with Au film. If only coralyne or the functional Au nanoparticles was present, the resonance wavelength was almost unchanged. It meant that there was no A-coralyne-A complex formed. Thus, this method could be used to detect coralyne, and the background was low. The results showed that the SPR sensor was highly sensitive, and the limit of detection was ca. 0.07 pmol/L (S/N＝3). It was about 5-6 orders of magnitude lower than those of fluorescent and colorimetric methods. Moreover, four kinds of small molecule drugs (berberine hydrochloride, penicillin, gentamicin sulfate, and 5-fluorouracil) were used as control to investigate the selectivity of this SPR biosensor. For penicillin, gentamicin sulfate, and 5-fluorouracil, all of the resonance wavelength shifts were lower than 0.4 nm. For high concentration of berberine hydrochloride, the resonance wavelength shifts were ca. 0.5-0.6 nm. The results showed that this SPR biosensor has good selectivity. This work may extend the application of SPR biosensors in drug discovery and development.