Acta Chimica Sinica ›› 2013, Vol. 71 ›› Issue (08): 1154-1160.

Article

一种新型酶生物燃料电池阳极构建及性能研究

1. a 湖南大学化学化工学院 化学生物传感与计量学国家重点实验室 长沙 410082;
b 湖南有色金属研究院 长沙 410015
• 投稿日期:2013-03-15 发布日期:2013-06-13
• 通讯作者: 陈金华, E-mail: chenjinhua@hnu.edu.cn; Tel.: 0086-0731-88821961; Fax: 0086-0731-88821848 E-mail:chenjinhua@hnu.edu.cn
• 基金资助:

项目受国家自然科学基金(No. 21275041)、湖南省自然科学基金(No. 12JJ2010)、高等学校博士学科点专项科研基金(No. 20110161110009)和长江学者和创新团队发展计划(No. IRT1238)资助.

Construction and Performance of a New Bioanode for Biofuel Cells

Zou Qionga, Liu Juana,b, Zhu Gangbinga, Zhang Xiaohuaa, Chen Jinhuaa

1. a State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082;
b Hunan Research Institute for Nonferrous Metals, Changsha 410015
• Received:2013-03-15 Published:2013-06-13
• Supported by:

Project supported by the National Natural Science Foundation of China (No. 21275041), Hunan Provincial Natural Science Foundation of China (No. 12JJ2010), the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20110161110009), and Program for Changjiang Scholars and Innovative Research Team in University (No. IRT1238).

Acid-treated carbon nanotubes (CNTs) were functionalized by β-cyclodextrin (CD) through the van der Waals force between them. It is well-known that CD is toroidal in shape with a hydrophobic inner cavity and a hydrophilic exterior. Based on the recognition of CD to ferrocene (Fc), a water-soluble complex, CNTs-β-CD-Fc, was prepared. Glucose oxidase (GOD) was mixed with CNTs-β-CD-Fc and crosslinked with glutaraldehyde to form enzyme polymer. Then the GOD/CNTs-β-CD-Fc composite was coated on the glassy carbon (GC) electrode and the GOD/CNTs-β-CD-Fc/GC bioanode was obtained. The prepared CNTs-β-CD and CNTs-β-CD-Fc were characterized by thermal gravimeritric analysis, fourier transform infrared spectroscopy and transmission electron microscopy. The electrocatalytic properties of the GOD/CNTs-β-CD-Fc/GC bioanode towards glucose oxidation were investigated by cyclic voltammetry. The results showed that under the same experimental conditions, the GOD/CNTs-β-CD/GC electrode (without Fc) had almost no catalytic currents for glucose oxidation and the GOD/CNTs-β-CD-Fc/GC bioanode had higher electrocatalytic activity towards glucose oxidation than the GOD/CNTs-Fc/GC electrode. The long-term cycle stability of the developed GOD/CNTs-β-CD-Fc/GC bioanode was also evaluated by cyclic voltammetry and the results showed that the GOD/CNTs-β-CD-Fc/GC bioanode had much better stability than the GOD/CNTs-Fc/GC electrode. Taking the commercial E-TEK Pt/C modified GC electrode as the cathode, the maximum power density of the glucose/O2 biofuel cell (EBFC) based on the GOD/CNTs-β-CD-Fc/GC anode was 33 μW·cm-2 (at 0.18 V), almost 3 times higher than that of the EBFC based on the GOD/CNTs-Fc/GC anode (11.7 μW·cm-2 at 0.16 V). The stability of the developed EBFC was also investigated by monitoring the change of the open circuit potential (OCP) of EBFC. After continuous operation for 9 h, the developed EBFC (the GOD/CNTs-β-CD-Fc/GC electrode as the bioanode) remained 92% of initial OCP, suggesting the good operating stability. The proposed strategy for bioanode preparation may provide a new way for the development of glucose/O2 EBFC with good perfomance and high stability.