Acta Chimica Sinica ›› 1996, Vol. 54 ›› Issue (4): 413-416. Previous Articles    

Original Articles

用高效毛细管电泳测定二(2'-脱氧-2'-氟)嘧啶核糖核苷酸对蛇毒磷酸二酯酶的稳定性

王政甫;陈耀全   

  1. 中国科学院上海有机化学研究所;中国科学院生命有机化学国家重点实验室
  • 出版日期:1996-04-15 发布日期:1996-04-15

High performance capillary electrophoresis as monitor for determination of stability of di(2'-deoxy-2'-fluoro) pyrimidinenucleosidephosphates to snake venom phosphodiesterase

WANG ZHENGFU;CHEN YAOQUAN   

  • Online:1996-04-15 Published:1996-04-15

High performance capillary electrophoresis (HPCE) has been used as monitor for the determination of stability of di(2'-deoxy-2'-fluoro) cytidine phosphate and di(2'-deoxy-2'-fluoro) uridine phosphate to snake venom phosphodiesterase (SVPDE). This seems to be a good method for monitoring the enzymatic reactions of moderate reaction rate.

Key words: STABILITY, PYRIMIDINE P, CATALYSIS, RIBONUCLEOTIDE

CLC Number: