Acta Chimica Sinica ›› 2003, Vol. 61 ›› Issue (9): 1455-1460. Previous Articles     Next Articles

Original Articles

两种新稀土双核配合物的合成、表征及其对磷酸二酯键模型物(BDNPP)和DNA的作用研究

杨频;周春琼   

  1. 山西大学分子科学研究所
  • 出版日期:2003-09-15 发布日期:2003-09-15

Synthesis and Characterization of Two New Rare-earth Complexes and Their Research for Cleaving an Activated Phosphate Diester BDNPP and DNA

Yang Pin;Zhou Chunqiong   

  1. Institute of Molecular Science, Shanxi University
  • Online:2003-09-15 Published:2003-09-15

[Eu_2(bbimp) (CH_3COO) (CH_3CH_2O)_2(CH_3CH_2OH)] (C1O_4)_2(A) and [Nd_2(bbimp) ( CH_3COO)-(CH_3CH_2O)_2(CH_3CH_2OH)](C1O_4)_2(B) were synthesized and characterized. The kinetic studies on the hydrolysis of BDNPP by complexes were carried out by UV-vis methods. The two reactions obey the second-order when the complexes (2.5 * 10~(-5) mol/L ) cleaved BDNPP (2.5 * 10~(-5) mol/L) . The rate constants and the half-time at 25 X. and pH 7.26 for the hydrolysis of BDNPP are 2479 ( mol/L) ~(-1) · min~(-1), 16.1 min in A , and 1678 ( mol/L) ~(- 1) · min~(-1), 23 .8 min in B. They are more reactive than that Eu~(3+) and Nd~(3+) for cleaving BDNPP. Furthermore, A or B is more reactive at 37 ℃ than at 25 ℃ for cleaving BDNPP. The pH-lgK for the two hydrolytic reactions were approximated with a linear equation at 25 ℃ in the pH 6.50 ~ 8.50. The investigation on the interaction of both complexes and CT DNA indicates that the absorbance value and the wavelength number at the maximum peak of GT DNA are reduced and red- shifted while the intensity of fluorescene spectra of EB-DNA is gradually weaken. The two complexes are capable of promoting cleavage of pBR322 DNA at 50℃ and pH 8.0.

Key words: RARE EARTH METAL COMPLEX, DINUCLEAR COMPLEX, PHOSPHATES, HYDROLYSIS, DNA, UV, REACTION KINETICS, EUROPIUM COMPLEX, NEODYMIUM COMPLEX

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