Study on the Interaction between Hematoxylin and DNA by Spec-trometry

Acta Chimica Sinica ›› 2007, Vol. 65 ›› Issue (2): 140-146. Previous Articles Next Articles

Original Articles

苏木素与DNA相互作用的光谱研究

王兴明*, 黎泓波, 胡亚敏, 杨定明, 费丹

(西南科技大学材料科学与工程学院化学系绵阳 621010)

投稿日期:2006-06-23 修回日期:2006-07-20 发布日期:2007-01-28
通讯作者: 王兴明

Study on the Interaction between Hematoxylin and DNA by Spec-trometry

WANG Xing-Ming*; LI Hong-Bo; HU Ya-Min; YANG Ding-Ming; FEI Dan

(Department of Chemistry, Material Science and Engineering College, Southwest University of Science and Technology, Mianyang 621010)

Received:2006-06-23 Revised:2006-07-20 Published:2007-01-28
Contact: WANG Xing-Ming

Abstract

The interaction of hematoxylin (HE) with DNA was studied by using acridine orange (AO) as a probe. The results indicated that there was a complex of hematoxylin and DNA, which has been confirmed by absorption, fluorescence spectra and viscometry. The binary complex ratio of n_HE∶n_DNA＝3∶1, the binding constant of HE interacting with DNA is 5.96×10⁴ L/mol. At the same time, the influence of acidity, salt effect and temperature on the interaction of hematoxylin with DNA was studied. The results suggested that the intercalation and electrostatic binding should be the two major modes for interaction between hematoxylin and herring sperm DNA.

Key words: hematoxylin, acridine orange, DNA, action mode

Cite this article

WANG Xing-Ming*; LI Hong-Bo; HU Ya-Min; YANG Ding-Ming; FEI Dan. Study on the Interaction between Hematoxylin and DNA by Spec-trometry[J]. Acta Chimica Sinica, 2007, 65(2): 140-146.

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苏木素与DNA相互作用的光谱研究

Study on the Interaction between Hematoxylin and DNA by Spec-trometry

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