Acta Chimica Sinica ›› 2011, Vol. 69 ›› Issue (04): 438-444. Previous Articles     Next Articles

Full Papers

基于PoPD-MWCNTs-离子液体/纳米金的髓过氧化物酶免疫传感器

刘蓓,逯岭松,刘成桂,谢国明*   

  1. (重庆医科大学检验系 临床检验诊断学教育部重点实验室 重庆 400016)
  • 收稿日期:2010-07-06 修回日期:2010-09-10 出版日期:2011-02-28 发布日期:2010-10-21
  • 通讯作者: 谢国明 E-mail:guomingxie@cqmu.edu.cn
  • 基金资助:

    基于PoPD-MWCNTs-离子液体/纳米金的髓过氧化物酶免疫传感器的研究

Immunosensor for Myeloperoxidase Based on PoPD-MWCNTs-Ionic Liquid/Nanogold Modified Au Electrode

LIU Bei, LU Ling-Song, LIU Cheng-Gui, XIE Guo-Ming   

  1. (Key Laboratory of Ministry of Education of Clinical Laboratory Diagnostics, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016)
  • Received:2010-07-06 Revised:2010-09-10 Online:2011-02-28 Published:2010-10-21

A novel label-free amperometric immunosensor for the determination of myeloperoxidase (MPO) based on poly-o-phenylenediamine (PoPD)-multi-wall carbon nanotubes (MWCNTs)-ionic liquid/ gold nanoparticles (nanogold) modified gold electrode (Au) was constructed. Firstly, a PoPD/MWNTs composite modified Au electrode was prepared in situ electropolymerization using ionic liquid as the supporting electrolyte. Then gold nanoparticles were adsorbed onto the electrode surface through amino-groups on the composite membrane for immobilization of MPO antibody via amine-Au affinity. The modification process was characterized by cyclic voltammetry (CV) and scanning electron microscope (SEM). The factors possibly influenced the performance of the proposed immunosensor were studied in detail. Under optimal conditions, the obtained immunosensor exhibited good electrochemical behavior to MPO in a linear range of 0.25 to 350 ng/mL (r=0.9985), with a detection limit of 0.07 ng/mL. The immunosensor has advantages of good stability, regeneration, high sensitivity and specificity. Thus, the method can be used for clinical application of MPO analysis.

Key words: myeloperoxidase, immunosensor, ionic liquid, poly-o-phenylenediamine, multi-wall carbon nanotubes