Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (01): 58-64.DOI: 10.6023/A1106072 Previous Articles     Next Articles

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甘晓娟, 刘绍璞, 刘忠芳, 王亚琼, 崔志平, 胡小莉   

  1. 发光与实时分析教育部重点实验室, 西南大学化学化工学院, 重庆 400715
  • 收稿日期:2011-06-07 修回日期:2011-07-31 出版日期:2012-01-14 发布日期:2012-02-25
  • 通讯作者: 胡小莉
  • 基金资助:


Fluorescence Quenching Method for the Determination of Analgin and Metabolin with Some Aromatic Amino Acids as Probes

Gan Xiaojuan, Liu Shaopu, Liu Zhongfang, Wang Yaqiong, Cui Zhiping, Hu Xiaoli   

  1. Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715
  • Received:2011-06-07 Revised:2011-07-31 Online:2012-01-14 Published:2012-02-25
  • Supported by:

    Project supported by the National Natural Science Foundation of China(No.20875078).

In pH 3.2 Britton-Robinson(BR)buffer medium,analgin(ANG)and metabolin of 4-methylaminoantipyrine(MAA)and 4-acetylaminoantipyrine(AAA)can react with some aromatic amino acids such as tryptophane(Trp),tyrosine(Tyr)and phenylalanine(Phe)to form complexes,which lead to fluorescence quenching of above amino acids,and the maximum quenching wavelength is at 352 nm (ANG-Trp system),304 nm(ANG-Tyr,MAA-Tyr and AAA-Tyr system),284 nm(ANG-Phe system),respectively.Fluorescence quenching value(ΔF)is proportional to the concentration of ANG,MAA and AAA in a certain range.Fluorescence quenching methods for the determination of ANG,MAA and AAA have high sensitivity with the detection limits of 13.3 ng/mL(Trp-ANG system),15.8 ng/mL(Tyr-ANG system), 64.5 ng/mL(Phe-ANG system),150.0 ng/mL(MAA-Tyr system)and 230.8 ng/mL(AAA-Tyr system),re- spectively.The optimum conditions of the reaction,influencing factors and the effect of coexisting sub-stances are investigated,and the results show this method has a good selectivity.This method has been successfully applied to the quick determination of ANG in tablets and its metabolin in urine samples.Judging from the change of the absorption spectra,the effect of temperature and the Stern-Volmer plots,the quenching of fluorescence of amino acids by ANG is a static quenching process.Experimental results show that a 1:1 compound of ANG and amino acid is formed by electrostatic attraction and aromatic stacking interaction.

Key words: analgin, amino acids, 4-methylaminoantipyrine, 4-acetylaminoantipyrine, fluorescence quenching