关键词: 三甲川吲哚菁染料, 非离子水溶性基团, 荧光量子产率, 蛋白质标记, 细胞染色

A novel water-soluble asymmetric trimethine cyanine dye which contains one nonionic hydrophilic group was first synthesized, which was prepared from one-step synthesis with N-carboxybenzyl-2,3,3-trimethyl-3H-indol-5-sulfonic acid, N,N′-diphenylformamidine and 2,3,3-trimethyl-3H-indol-5-sulfonic acid with PEG. The pure title compound could be obtained by using the self-made C18 reversed-phase chromatographic column. The structure of the product was identified by NMR and HRMS. The spectra character and photostability of this dye were detected, protein labeling of albumin bovine serum (BSA) and cell stain with this dye were investigated. The results showed that the yield of the target compound could reach 73% by simple synthesis and purification, the fluorescence quantum yield was 0.3, the D/P of protein labeling was 1.87. The dye could effectively distinguish between the fixed cells and living cells, because of the significant differences in the staining results.

Key words: trimethine indocyanine, nonionic hydrophilic group, fluorescence quantum yield, protein labeling, cell stain