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有机化学
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有机化学 ›› 2011, Vol. 31 ›› Issue (11): 1811-1819. 上一篇    下一篇

研究论文

新的滇桂艾纳香寡糖BROS结构分析

许子竞1,3，林翠梧*1，廖敏富1,2   

  1. (1广西大学化学化工学院 南宁 530004)
    (2广西药用植物园 南宁 530023)
    (3广西亿康药业股份有限公司 柳州 545006)
  • 收稿日期:2010-07-21 修回日期:2011-03-21 发布日期:2011-06-02
  • 通讯作者: 林翠梧 E-mail:lincuiwu@163.com

Structure Analysis of a New Oligosaccharides BROS from Blumea riparia

Xu Zijing1,3 Lin Cuiwu*1 Liao Minfu1,2   

  1. (1 College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004)
    (2 Guangxi Medicinal Plants Botantical Garden, Nanning 530023)
    (3 Guangxi Ecan Pharmaceutical Co. LTD, Liuzhou 545006)
  • Received:2010-07-21 Revised:2011-03-21 Published:2011-06-02
  • Contact: Cui-wu LIN E-mail:lincuiwu@163.com

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1158

被引次数

8

以滇桂艾纳香枝叶为原料, 经陶瓷膜、有机膜分离, 醇沉、Sevag法除蛋白, DEAE-纤维素柱层析和SephadexG-10, SephadexG-50凝胶柱色谱纯化得到的BROS|经HPGPC检测分析表明, BROS为均一寡糖, MALDI-TOF-MS测定分子量为1314. 用IR, HPLC, 甲基化分析, GC-MS, NMR (1H NMR, 13C NMR, 1H-1HCOSY, HMQC和HMBC)等方法对BROS结构进行表征, 结果表明, 该寡糖由1个葡萄糖(α-D-Glcp)和7个果糖(β-D-Fruf)组成, 残基间以→2)Fruf-(1→链接, 分子末端链接为α-Glcp-(1→, →2)-β-D-Fruf, 推出BROS的结构式为: β-D-Fruf1-(2→1)-β-D-Fruf2-(2→1)- β-D-Fruf3-(2→1)-β-D-Fruf4-(2→1)-β-D-Fruf5-(2→1)-β-D-Fruf6-(2→1)-β-D-Fruf7-(2→1)-α-D-Glcp.

关键词: 寡糖(BROS), 提取纯化, 甲基化还原裂解, 核磁分析, 结构表征

Blumea riparia oligosaccharide (BROS) was obtained from branches of Blumea riparia by using ceramic and organic membrance separation, precipitation with ethanol, deproteination using Sevag method, and fractionation through DEAE-cellulose column, Sephadex G-10 and Sephadex G-50 Gel filtration chromatography. The purity and molecular weight of BROS were determined by HPGPC and MALDI-TOF-MS. The structure was characterized with monosaccharide composition analysis, methylation analysis, IR, GC-MS, 1D and 2D NMR analysis. The results showed that BROS was homogeneous oligo-saccharide and composed of one α-D-Glcp and seven β-D-Fruf. The molecular weight of BROS was 1314. The backbone of BROS had a consisting of 1,2-linked β-D-Fruf residues, α-D-Glcp-(1→ and β-D-Fruf-(2→ connecting each end of the molecular. Finally, the BROS structure was fixed on as: β-D-Fruf1-(2→1)-β- D-Fruf2-(2→1)-β-D-Fruf3-(2→1)-β-D-Fruf4-(2→1)-β-D-Fruf5-(2→1)-β-D-Fruf6-(2→1)-D-Fruf7-(2→1)-α-D-Glcp.

Key words: oligosaccharide (BROS), extraction and isolation, methylation reductive cleavage, NMR analysis, structure characterization