Chin. J. Org. Chem. ›› 2013, Vol. 33 ›› Issue (03): 573-580.DOI: 10.6023/cjoc201211033 Previous Articles     Next Articles



徐志远a,b,c, 李永军c,d, 史萍b, 王博婵b, 黄晓宇c   

  1. a 华东理工大学材料科学与工程学院 上海 200237;
    b 生物反应器工程国家重点实验室 上海 200237;
    c 中国科学院上海有机化学研究所 上海 200032;
    d 聚合物分子工程国家重点实验室(复旦大学) 上海 200043
  • 收稿日期:2012-11-16 修回日期:2012-12-01 发布日期:2012-12-07
  • 通讯作者: 史萍, 黄晓宇;
  • 基金资助:

    国家自然科学基金(Nos. 31100549, 21204098)、上海市青年科技启明星跟踪计划(No. 11QH1402800)和上海市生物医药科技重点(No. 10431903000)资助项目.

Functionalized Graphene Oxide as a Nanocarrier for Loading and Delivering of Oridonin

Xu Zhiyuana,b,c, Li Yongjunc,d, Shi Pingb, Wang Bochanb, Huang Xiaoyuc   

  1. a School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237;
    b The State Key Laboratory of Bioreactor Engineering, Shanghai 200237;
    c Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032;
    d State Key Laboratory of Molecular Engineering of Polymers (Fudan University), Shanghai 200043
  • Received:2012-11-16 Revised:2012-12-01 Published:2012-12-07
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos. 31100549, 21204098), the Shanghai Rising Star Program (No. 11QH1402800), and the Shanghai Scientific and Technological Innovation Project (No. 10431903000).

Graphene oxide (GO) was prepared by modified Hummers method firstly. In order to improve its water solubility and biocompatibility, 6-armed PEG was grafted onto GO via an amidation process. The size of GO-PEG was less than 250 nm and stability test indicated excellent dispersibility of GO-PEG in water and PBS buffer. Furthermore, oridonin, a widely used cancer chemotherapy drug, is adsorbed onto GO-PEG via blending. The drug loading ratio was determined to be as high as 105%, which was much higher than other common drug carriers. A549 lung cancer cell and MCF-7 breast cancer cell were chosen to study the cytotoxicity of GO-PEG/oridonin, GO-PEG, and free oridonin. The results demonstrated that GO-PEG did not show obvious toxicity (relative cell viability>85%), even cultivated for 48 h at a relatively high concentration of 100 mg/L. Compared to oridonin, the GO-PEG/oridonin nanocarrier shows higher cytotoxicity in A549 and MCF-7 cells.

Key words: graphene oxide, oridonin, drug delivery