Preparation and Photocytotoxicity in vitro of Oridonin-porphyrinchitosan Microspheres

doi:10.6023/cjocx201607022

Chin. J. Org. Chem. ›› 2017, Vol. 37 ›› Issue (3): 759-766.DOI: 10.6023/cjocx201607022 Previous Articles Next Articles

Notes

冬凌草甲素卟啉壳聚糖微球的制备及体外光动力抗肿瘤活性研究

何洁^a, 曹泳^a, 袁强^b

a 浙江中医药大学生命科学学院杭州 310053;
b 浙江中医药大学药学院杭州 310053

收稿日期:2016-07-14 修回日期:2016-10-21 发布日期:2016-11-22
通讯作者: 袁强,E-mail:yuanqiang0825@sina.com E-mail:yuanqiang0825@sina.com
基金资助:
2017年浙江省中医药科技计划（No.2017ZB027）资助项目.

Preparation and Photocytotoxicity in vitro of Oridonin-porphyrinchitosan Microspheres

He Jie^a, Cao Yong^a, Yuan Qiang^b

a College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053;
b College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053

Received:2016-07-14 Revised:2016-10-21 Published:2016-11-22
Contact: 10.6023/cjocx201607022 E-mail:yuanqiang0825@sina.com
Supported by:
Project supported by the Zhejiang Science and Technology Program of Traditional Chinese Medicine (No. 2017ZB027).

1. Preparation and Photocytotoxicity in vitro of Oridonin-porphyrinchitosan Microspheres.PDF(764KB)

Abstract

A emulsion crosslinking method to prepare oridonin-porphyrin-chitosan microspheres with oridonin and 5-p-(6-bromohexylaminophenyl)-10,15,20-triphenylporphyrin-chitosan (BHP-CS) is reported, which in turn was synthesized from 5-p-(6-bromo-hexylaminophenyl)-10,15,20-triphenylporphyrin (BHP) and chitosan. The porphyrin grafting rate of BHP-CS reached 30.80% on average, the drug loading rate and entrapment rate of the prepared microspheres reached 12.41% and 8.72% measured by HPLC, and the accumulated release rate was 81.74% in 48 h in vitro. The thiazolyl blue tetrazolium bromide (MTT) method was used to evaluate the photocytotoxicities of these derivatives against MCF-7 cells. The results revealed that oridonin-porphyrin-chitosan microspheres showed high photocytotoxicity in concentrations of 25, 50 and 100 μmol/mL against MCF-7 cells, and the inhibit ratio of MCF-7 cells under light irradiation for 30 min were (31.55±1.70)%, (71.03±0.76)% and (82.74±0.38)%, respectively.

Key words: oridonin, porphyrin, microspheres, photocytotoxicity, antitumor

Cite this article

He Jie, Cao Yong, Yuan Qiang. Preparation and Photocytotoxicity in vitro of Oridonin-porphyrinchitosan Microspheres[J]. Chin. J. Org. Chem., 2017, 37(3): 759-766.

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冬凌草甲素卟啉壳聚糖微球的制备及体外光动力抗肿瘤活性研究

Preparation and Photocytotoxicity in vitro of Oridonin-porphyrinchitosan Microspheres

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