Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (4): 1038-1042.DOI: 10.6023/cjoc201908033 Previous Articles     Next Articles


张坤a, 谢向前b, 史海霞a, 沈月毛a,b, 王浩鑫a   

  1. a 山东大学微生物技术研究院 微生物技术国家重点实验室 青岛 266237;
    b 山东大学药学院 天然产物化学生物学教育部重点实验室 济南 250012
  • 收稿日期:2019-08-26 修回日期:2019-11-08 发布日期:2020-05-06
  • 通讯作者: 沈月毛, 王浩鑫;
  • 基金资助:

ortho-Dialkyl-Substituted Aromatic Acids from Verrucosispora sp. NS0172

Zhang Kuna, Xie Xiangqianb, Shi Haixiaa, Shen Yuemaoa,b, Wang Haoxina   

  1. a State Key Laboratory of Microbial Technology, Institute of Microbial Technology, Shandong University, Qingdao 266237;
    b Key Laboratory of Chemical Biology(Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012
  • Received:2019-08-26 Revised:2019-11-08 Published:2020-05-06
  • Supported by:
    Project supported by the National Natural Science Foundation of China (Nos. 31570039, 81530091), and the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT_17R68).

Two new ortho-dialkyl-substituted aromatic acids 1 and 2 were isolated from Verrucosispora sp. NS0172. The chemical structures of 1 and 2 were determined by spectroscopic methods including 1D- and 2D-NMR and HR-ESIMS experiments. The cytotoxicity of compounds 1 and 2 was evaluated by methyl thiazolyl tetrazolium (MTT) assay, and compound 1 showed potent antiproliferative activity against human hepatocellular carcinoma SMMC-7721 (IC50 7.74 μmol·L-1). Compounds 1 and 2 were tested for the antimicrobial and antifungal activities by filter paper disc diffusion assay, and both of them showed no evident activities at a dose of 40 μg/disc. This study is the first report of discovering of ortho-dialkyl-substi-tuted aromatic acids from Verrucosispora, which sets the foundation for future biosynthetic study of this class of natural products in Verrucosispora.

Key words: verrucosispora, actinomycetes, natural product, ortho-dialkyl-substituted aromatic acids