The optical resolution of racemic mixtures of thirteen 2, 3- epoxideamides with different substituting group was achieved with HPLC by using Chiralcel OD and Chiralpak AD as chiral stationary phase and hexane/2-propanol mixtures with different concentrations as eluent. The chromatographic parameters of theseracemates on AD and OD columns were examined. The results showed that the chiral recognization process was the sum of all the possible chiral interactions (the dipole-dipole, hydrogen bond and π-π interactions) and achiral interaction (such as steric effect) between 2, 3- epoxideamides and the chiral stationary phase. This method had been applied to identify the optical purity of 2, 3-epoxideamides.

Key words: ELUENT, CHIRAL REAGENT, ISOPROPANOL, AMIDES P, HEXANE, EPOXIDE, ENANTIOMORPH, STATIONARY PHASE, MESOTOMY, HIGH-SPEED LIQUID CHROMATOGRAPHY