Functionalized Graphene Oxide as a Nanocarrier for Loading and Delivering of Eriocalyxin B

doi:10.6023/cjoc201306011

Chin. J. Org. Chem. ›› 2013, Vol. 33 ›› Issue (10): 2162-2168.DOI: 10.6023/cjoc201306011 Previous Articles Next Articles

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功能化石墨烯负载毛萼乙素抗肿瘤制剂的研究

徐志远^a,b,c, 李永军^b, 史萍^a, 王博婵^a, 黄晓宇^b

a 华东理工大学生物反应器工程国家重点实验室上海 200237;
b 中国科学院上海有机化学研究所上海 200032;
c 华东理工大学材料科学与工程学院上海 200237

收稿日期:2013-06-07 修回日期:2013-06-18 发布日期:2013-06-25
通讯作者: 史萍, 黄晓宇 E-mail:ship@ecust.edu.cn;xyhuang@mail.sioc.ac.cn
基金资助:
国家自然科学基金(Nos. 31100549, 21204098);上海市生物医药科技重点(No. 10431903000)和国家重点实验室专项经费(No. 2060204)资助项目

Functionalized Graphene Oxide as a Nanocarrier for Loading and Delivering of Eriocalyxin B

Xu Zhiyuan^a,b,c, Li Yongjun^b, Shi Ping^a, Wang Bochan^a, Huang Xiaoyu^b

a State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237;
b Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032;
c School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237

Received:2013-06-07 Revised:2013-06-18 Published:2013-06-25
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 31100549, 21204098), the Shanghai Scientific and Technological Innovation Project (No. 10431903000) and the National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204).

Graphene oxide (GO) was firstly prepared by modified Hummers method. In order to improve its water solubility and biocompatibility, 6-arm PEG was grafted to GO via a facile amidation reaction. The size of obtained GO-PEG was less than 250 nm. Stability test indicated the good dispersibility of GO-PEG in water and PBS buffer. Furthermore, eriocalyxin B, a widely used cancer chemotherapy drug, is adsorbed onto GO-PEG via physical blending with a drug loading ratio of 18.8% obtained by UV spectrum. Lung cancer cell A549 and breast cancer cell MCF-7 were selected to study the cytotoxicity of GO-PEG/eriocalyxin B, GO-PEG, and free eriocalyxin B. The results demonstrated that GO-PEG nano-carrier possessed low toxicity (relative cell viability>85%), even cultivated for 48 h at a relatively high concentration of 100 mg/L. Compared to pure drug, GO-PEG/eriocalyxin B nanocarrier shows higher cytotoxicity in A549 and MCF-7 cells.

Key words: graphene oxide, eriocalyxin B, drug delivery, cell viability

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Xu Zhiyuan, Li Yongjun, Shi Ping, Wang Bochan, Huang Xiaoyu. Functionalized Graphene Oxide as a Nanocarrier for Loading and Delivering of Eriocalyxin B[J]. Chin. J. Org. Chem., 2013, 33(10): 2162-2168.

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功能化石墨烯负载毛萼乙素抗肿瘤制剂的研究

Functionalized Graphene Oxide as a Nanocarrier for Loading and Delivering of Eriocalyxin B

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