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化学学报
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化学学报 ›› 2012, Vol. 0 ›› Issue (04): 479-484.DOI: 10.6023/A1108303 上一篇    下一篇

研究论文

肝素化丝素支架作为骨形态发生蛋白-2 缓释载体的研究

马寅孙, 蔡玉荣, 刘琳, 姚菊明   

  1. 浙江理工大学材料与纺织学院 先进纺织材料与制备技术教育部重点实验室 杭州 310018
  • 投稿日期:2011-08-30 修回日期:2011-10-10 发布日期:2011-11-28
  • 通讯作者: 姚菊明
  • 基金资助:

    国家自然科学基金(Nos. 21041003, 51172207)资助项目.

Heparinized Silk Fibroin Scaffolds as the Sustained Release Carrier of Bone Morphogenetic Protein-2

Ma Yinsun, Cai Yurong, Liu Lin, Yao Juming   

  1. The Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018
  • Received:2011-08-30 Revised:2011-10-10 Published:2011-11-28
  • Supported by:

    Project supported by the National Natural Science Foundation of China (Nos. 21041003 and 51172207).

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骨形态发生蛋白-2(BMP-2)的缓释载体一直是骨组织工程中的研究热点. 本研究通过化学改性制备了两种肝素化丝素支架, 并浸渍吸附BMP-2, 研究了BMP-2 在不同丝素支架样品上的吸附能力、体外释放性能及其对人骨肉瘤细胞MG-63 碱性磷酸酶活性(ALP)的影响. 结果表明, 肝素化丝素支架对BMP-2 具有较强的吸附能力, 并能保持其体外缓慢释放性能; MG-63 细胞在肝素化支架上生长状态良好, 并具有显著的增殖能力, 负载BMP-2 后的肝素化支架能显著促进MG-63 细胞的分化. 因此, 肝素化丝素支架是一种较理想的BMP-2 缓释载体.

关键词: 丝素支架, BMP-2, 碱性磷酸酶, 缓释

Sustained release carrier for the bone morphogenetic protein-2 (BMP-2) is currently a hot subject of research on the bone tissue engineering. In this work, two kinds of heparinized silk fibroin scaffolds were prepared. The binding ability to BMP-2, in vitro sustained release property and the alkaline phosphatase (ALP) activity of the scaffolds were investigated. Results showed that the heparinized silk fibroin scaffolds could bind BMP-2 well and sustained release the BMP-2. The scaffolds with BMP-2 could improve the MG-63 cell growth and cell differentiation with high ALP activity. So the heparinized silk fibroin scaffolds could be an ideal sustained release carrier for BMP-2.

Key words: silk fibroin scaffold, BMP-2, alkaline phosphatase, sustained release