基于便携拉曼光谱仪和双层纸芯片的头发皮质醇超高灵敏检测

doi:10.6023/A16110598

化学学报 ›› 2017, Vol. 75 ›› Issue (4): 355-359.DOI: 10.6023/A16110598 上一篇下一篇

研究通讯

基于便携拉曼光谱仪和双层纸芯片的头发皮质醇超高灵敏检测

高志刚^a, 郑婷婷^a, 邓九^a, 李晓瑞^a, 曲玥阳^a, 陆瑶^b, 刘婷娇^c, 罗勇^a, 赵伟杰^a, 林炳承^a

a 大连理工大学制药科学与技术学院大连 116024;
b 大连化学物理研究所生物技术部大连 116023;
c 大连医科大学口腔医学院大连 116044

投稿日期:2016-11-11 发布日期:2017-02-23
通讯作者: 罗勇,E-mail:yluo@dlut.edu.cn;Tel.:+86-411-84986360;Fax:+86-411-84986360 E-mail:yluo@dlut.edu.cn
基金资助:
项目受国家自然科学基金（No.21675017）资助.

Ultrasensitive Detection of Hair Cortisol Based on Portable Raman Spectrometer and Double-layer Paper Microdevice

Gao Zhigang^a, Zheng Tingting^a, Deng Jiu^a, Li Xiaorui^a, Qu Yueyang^a, Lu Yao^b, Liu Tingjiao^c, Luo Yong^a, Zhao Weijie^a, Lin Bingcheng^a

a School of Pharmaceutical Science and Technology, Dalian University of Technology, Dalian 116024;
b Biological Division, Dalian Institute of Chemical Physics, Dalian 116023;
c College of Stomatology, Dalian Medical University, Dalian 116044

Received:2016-11-11 Published:2017-02-23
Contact: 10.6023/A16110598 E-mail:yluo@dlut.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (No. 21675017).

文章分享

1. 基于便携拉曼光谱仪和双层纸芯片的头发皮质醇超高灵敏检测.PDF(1568KB)

摘要/Abstract

人头发中的皮质醇是反映现代人长期精神压力累积的主要临床标记物.建立了一种人发皮质醇的超高灵敏检测方法.方法基于表面增强拉曼光谱、免疫分析和双层纸芯片.纸芯片的第一层用于滤除人发提取液中的头发残渣（样品前处理），第二层用于实施竞争性免疫反应和拉曼检测.固定在纸表面的皮质醇抗原和游离的头发皮质醇竞争结合能产生拉曼信号的皮质醇单克隆抗体，通过检测结合在纸表面的皮质醇抗原-抗体复合物的拉曼信号进行游离头发皮质醇的定量.在便携式拉曼光谱仪上，皮质醇抗体的拉曼信号经过优化，方法的检测限可以达到1 pg/mL，平行测定相对标准偏差为8.38%（n=6）.进行了两例实际样品检测，液质检测结果分别为0.771和0.153 ng/mL，本方法检测结果分别为0.63和0.247 ng/mL，两种方案的结果在一个数量级，证明了方法的实用性.利用此方法，一块芯片可以同时测定48个样品，专属性和准确性很好，特别适合于人群精神压力的普查.

关键词: 头发皮质醇, 表面增强拉曼光谱, 纸芯片, 便携式拉曼光谱仪, 竞争性免疫

Cortisol in the human hair is a clinical biomarker of long-term social-and-work-related mental stress, which is of high morbidity rate in the current modern society. This study developed a sensitive hair cortisol assay, featuring sur-face-enhanced Raman spectroscopy, immunoreaction, and a double-layer paper microdevice. The first layer of the paper mi-crodevice was used to remove the hair residue in the hair extract by filtration (sample pretreatment). The second layer was used for competitive immunoreaction and detection. Standard cortisol antigen immobilized in the second layer and the free cortisol in hair extract competed to bind the spiked Raman-active cortisol monoclonal antibody solution. The hair cortisol can be quantitated by the intensity of Raman signal of monoclonal antibody bound on the paper. We found the Raman signal decreased as the cortisol concentration increases in hair samples. The relative Raman intensity measured was linearly proportional to the logarithmic value of the cortisol concentration in hair samples we measured. The detection of limit (LOD) was 1 pg/mL with the portable Raman spectrometer. The RSD of measurement was 8.38% (n=6). In addition, we used LC-MS to measure two real samples as a comparison with our method as above. The results are 0.771 and 0.153 ng/mL by LC-MS method and 0.63 and 0.247 ng/mL by the proposed method. It can be observed that the results are in same order, demonstrating the validity of the proposed method. In addition, 48 samples can be measured in a single chip. These results showed that this method is sensitive, specific, and suitable for large-scale screening of hair cortisol samples.

Key words: hair cortisol, SERS, paper microdevice, portable Raman spectrometer, competitive immunoassay

引用此文

高志刚, 郑婷婷, 邓九, 李晓瑞, 曲玥阳, 陆瑶, 刘婷娇, 罗勇, 赵伟杰, 林炳承. 基于便携拉曼光谱仪和双层纸芯片的头发皮质醇超高灵敏检测[J]. 化学学报, 2017, 75(4): 355-359.

Gao Zhigang, Zheng Tingting, Deng Jiu, Li Xiaorui, Qu Yueyang, Lu Yao, Liu Tingjiao, Luo Yong, Zhao Weijie, Lin Bingcheng. Ultrasensitive Detection of Hair Cortisol Based on Portable Raman Spectrometer and Double-layer Paper Microdevice[J]. Acta Chim. Sinica, 2017, 75(4): 355-359.

导出引用 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

分享此文

参考文献

[1] Ito, N.; Ito, T.; Kromminga, A.; Bettermann, A.; Takigawa, M.; Kees, F.; Straub, R. H.; Paus, R. FASEB J. 2005, 19, 1332.
[2] Van Uum, S. H. M.; Sauve, B.; Fraser, L. A.; Morley-Forster, P.; Paul, T. L.; Koren, G. STRESS 2008, 11, 483.
[3] Manenschijn, L.; Koper, J. W.; Lamberts, S. W. J.; van Rossum, E. F. C. Steroids 2011, 76, 1032.
[4] Davenport, M. D.; Tiefenbacher, S.; Lutz, C. K.; Novak, M. A.; Meyer, J. S. Gen. Comp. Endocrinol. 2006, 147, 255.
[5] Raul, J. S.; Cirimele, V.; Ludes, B.; Kintz, P. Clin. Biochem. 2004, 37, 1105.
[6] Accorsi, P. A.; Carloni, E.; Valsecchi, P.; Viggiani, R.; Garnberoni, M.; Tarnanini, C.; Seren, E. Gen. Comp. Endocrinol. 2008, 155, 398.
[7] Lu, Y.; Shi, W. W.; Jiang, L.; Qin, J. H.; Lin, B. C. Electrophoresis 2009, 30, 1497.
[8] Lu, Y.; Shi, W. W.; Qin, J. H.; Lin, B. C. Anal. Chem. 2010, 82, 329.
[9] Klasner, S. A.; Price, A. K.; Hoeman, K. W.; Wilson, R. S.; Bell, K. J.; Culbertson, C. T. Anal. Bioanal. Chem. 2010, 397, 1821.
[10] Ge, S. G.; Ge, L.; Yan, M.; Song, X. R.; Yu, J. H.; Huang, J. D. Chem. Commun. 2012, 48, 9397.
[11] Vella, S. J.; Beattie, P.; Cademartiri, R.; Laromaine, A.; Martinez, A. W.; Phillips, S. T.; Mirica, K. A.; Whitesides, G. M. Anal. Chem. 2012, 84, 2883.
[12] Yan, C.; Yu, S.; Jiang, Y.; He, Q.; Chen, H. Acta Chim. Sinica 2014, 72, 1099. (严春芳, 余思扬, 蒋艳, 何巧红, 陈恒武, 化学学报, 2014, 72, 1099.)
[13] Zhao, L.; Yan, H. Acta Chim. Sinica 2012, 70, 1104. (赵联朝, 闫宏涛, 化学学报, 2012, 70, 1104.)
[14] Wang, Y.; Yan, B.; Chen, L. Chem. Rev. 2013, 113, 1391.
[15] Zhang, W.; Li, B.; Chen, L.; Wang, Y.; Gao, D.; Ma, X.; Wu, A. Anal. Methods 2014, 6, 2066.
[16] Li, B.; Zhang, W.; Chen, L.; Lin, B. Electrophoresis 2013, 34, 2162.
[17] Fu, X.; Cheng, Z.; Yu, J.; Choo, P.; Chen, L.; Choo, J. Biosens. Bioelectron. 2016, 78, 530.
[18] Dou, B.; Luo, Y.; Chen, X.; Shi, B.; Du, Y. G.; Gao, Z. G.; Zhao, W. J.; Lin, B. C. Electrophoresis 2015, 36, 485.
[19] Wang, G. F.; Lipert, R. J.; Jain, M.; Kaur, S.; Chakraboty, S.; Torres, M. P.; Batra, S. K.; Brand, R. E.; Porter, M. D. Anal. Chem. 2011, 83, 2554.
[20] Zhang, Z.; Liu, R.; Xu, D.; Liu, J. Acta Chim. Sinica 2012, 70, 1686(in Chinese). (张宗绵, 刘睿, 徐敦明, 刘景富, 化学学报, 2012, 70, 1686.)

基于便携拉曼光谱仪和双层纸芯片的头发皮质醇超高灵敏检测

Ultrasensitive Detection of Hair Cortisol Based on Portable Raman Spectrometer and Double-layer Paper Microdevice

PDF

补充材料

可视化

被引次数

摘要/Abstract

引用此文

分享此文

参考文献

相关文章 15

编辑推荐

相关统计

本文评价

[1]	易荣楠, 吴燕. 表面增强拉曼光谱技术在microRNA检测中的研究进展[J]. 化学学报, 2021, 79(6): 694-704.
[2]	袁宏宇, 徐敏敏, 姚建林. 电化学SPR协同催化对氯苯硫酚界面反应的SERS研究[J]. 化学学报, 2021, 79(12): 1481-1485.
[3]	杨晶亮, 杨伟民, 林嘉盛, 汪安, 徐娟, 李剑锋. 电场强度对等离激元诱导热电子的影响[J]. 化学学报, 2020, 78(7): 670-674.
[4]	倪宇欣, 张晨杰, 袁亚仙, 徐敏敏, 姚建林. 纳米ZnO的表面增强拉曼散射效应来源研究[J]. 化学学报, 2019, 77(7): 641-646.
[5]	左方涛, 徐威, 赵爱武. 基于功能化Fe₃O₄@Ag纳米粒子快速检测Hg²⁺的SERS方法[J]. 化学学报, 2019, 77(4): 379-386.
[6]	刘娇, 孙海龙, 印璐, 袁亚仙, 徐敏敏, 姚建林. 微流控芯片结合表面增强拉曼光谱实时监测α-苯乙醇的微量合成反应[J]. 化学学报, 2019, 77(3): 257-262.
[7]	王猛, 闫昕, 韦德泉, 梁兰菊, 王岳平. Au/Ag复合纳米笼在表面增强拉曼光谱中的应用[J]. 化学学报, 2019, 77(2): 184-188.
[8]	程劼, 王培龙, 苏晓鸥. 表面增强拉曼光谱检测二噁英类化合物研究进展[J]. 化学学报, 2019, 77(10): 977-983.
[9]	马超, 武佳炜, 朱琳, 韩晓霞, 阮伟东, 宋薇, 王旭, 赵冰. g-C₃N₄/Ag纳米复合材料表面增强拉曼基底对婴幼儿糖果中的罗丹明B的痕量检测[J]. 化学学报, 2019, 77(10): 1024-1030.
[10]	焦岑蕾, 王炜, 刘娇, 袁亚仙, 徐敏敏, 姚建林. 表面增强拉曼光谱与高效液相色谱联用技术在Suzuki偶联反应实时[J]. 化学学报, 2018, 76(7): 526-530.
[11]	张晨杰, 张婧, 林洁茹, 金琦, 徐敏敏, 姚建林. 金纳米粒子单层膜表面SPR催化反应的原位SERS研究[J]. 化学学报, 2017, 75(9): 860-865.
[12]	翟晨, 彭彦昆, 李永玉, 徐田锋. 基于表面增强拉曼光谱的苹果毒死蜱残留无损检测方法[J]. 化学学报, 2015, 73(11): 1167-1172.
[13]	赵刘斌, 黄逸凡, 吴德印, 任斌. 对氨基苯硫酚分子的表面增强拉曼光谱及等离激元光催化反应[J]. 化学学报, 2014, 72(11): 1125-1138.
[14]	严春芳, 余思扬, 蒋艳, 何巧红, 陈恒武. 基于等离子体技术制作微流控纸芯片及其在血糖检测中的应用研究[J]. 化学学报, 2014, 72(10): 1099-1104.
[15]	梅金华, 徐敏敏, 袁亚仙, 杨凤珠, 顾仁敖, 姚建林. 离子液体中Fe电极界面结构的SERS光谱研究[J]. 化学学报, 2013, 71(07): 1059-1063.