化学学报 ›› 1988, Vol. 46 ›› Issue (11): 1125-1133. 上一篇    下一篇

研究论文

计算机辅助的羧肽酶法测定多肽羧端顺序

汪猷;孙小俭;钱诚;钱瑞卿;张伟君;顾天爵   

  1. 中国科学院上海有机化学研究所;上海医科大学生化教研室
  • 发布日期:1988-11-15

The computer-assisted carboxypeptidase method for C-terminal sequencing of proteins and polypeptides

WANG YOU;SUN XIAOJIAN;QIAN CHENG;QIAN RUIQING;ZHANG WEIJUN;GU TIANJUE   

  • Published:1988-11-15

在初步用计算机辅助的羧肽酶法测定了天花粉蛋白C-端顺序的基础上, 进一步设计了两个计算机程序-DPS程序和CPA程序. 用合成小肽和天然的肽对这两个计算机程序进行模型实验证明, 运用这两个程序能分别满意地从羧肽酶的酶解动力学曲线中获得重要的C-端顺序信息, 并测定了天花粉蛋白分子中未知肽段CB-3的C-端顺序为: -SerAlaSerAlaLeuHserOH, 这一顺序后来已经其他实验结果所证实. 本法不仅使C-端顺序测定延长至七个氨基酸, 而且还基本上解决了多肽或蛋白质含有多种多次重复氨基酸残基的C-端顺序测定.

关键词: 多肽, 天花粉, 氨基酸顺序分析, 羧肽酶, 计算机辅助设计, 蛋白质, 程序系统

On study of computer-assisted carboxypeptidase method for C-terminal sequence determination of proteins or peptides, two computer programs, DPS and CPA, were successfully developed on VAX-11/780 computer and tested with some synthetic peptides and degradation fragments of natural protein as model substrates. The C-terminal sequence of CB-3, one of the CNBr degradation fragments of trichosanthin was determine by this method as: -SerAlaSerAlaAlaLeuHseOH, which was later confirmed by other ways of sequencing. This method is not only able to extend the C-terminal sequencing up to 7 amino acid residues, but also useful for determing the C-terminal sequence with some amino acids residues appearing in repetition.

Key words: POLYPEPTIDE, RADIX TRICHOSANTHIS, AMINO ACID SEQUENCE ANALYSIS, CARBOXYL PEPTIDASE, COMPUTER AIDED DESIGN, PROTEIN, PROGRAMMING SYSTEMS

中图分类号: